I need to dissolve a few fatty acids of C10 to C20 such as myristic, stearic and arachidic acid for a PRO-DNA binding reaction to carry out an EMSA. I tried with DMSO, where I dissolved the acids in 100% DMSO and used in the reaction directly. I checked the effect of DMSO in my binding reaction and it didn't interfere with the binding. Are there any good suggestions to dissolve and dilute fatty acids which are more suitable for a PRO-DNA binding reaction?
Chinaza Godswill Awuchi
Kindly check https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2009.06757.x
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Chinaza Godswill Awuchi
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912384/
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Karima Galal Abdel hameed
Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells
- February 2008
- Methods in Molecular Biology 456:231-47
- DOI: 10.1007/978-1-59745-245-8-17
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