We use cells as doing subculture (ex: cancer cells). However I don't know of the timing changing the cells.
When is the timing changing the cell ?
Thanks for your opinion.
Sadly, we use the self-developed cells, so not information at ATCC web.
Are you meaning that when doubling time is abnormal, change the cells ?
Hi. I understand the Your "changing the cells" means "sub-culturing".
If so, as already suggested, You would have to count the cells on a daily basis when they are proliferating in vitro, and see when the cells reach the so-called "plateu". Then, in Your next experiments, You have sub-culture Your cells before they reach the plateu phase.
Regards
Stefano
Dear falone,
Thank you for your opinion. However, I don't mean changing the cells as sub-culturing. I meant what change the new cells thawed. By any chance, I want your suggestion about this.
Regards,
Daiha Shin
Dear Daiha Shin,
I do not know if I have understood your question, but I'll try to give an answer.
Primary cells undergo senescence after 12-15 passages. After that passage all results could be altered. So the best thing should be to freeze several cell's aliquotes after passage 3/4 (almost 250000/400000 per ml).
Stable cell culture lines undergo senescence after about 20/25 passages.
Did I answer to your question?
Regards,
MArco
Dear Marco,
thank you for your opinion.
This is what I want.
Regards,
Daiha Shin
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