How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to analyse data without control group in meta-analysis?

I am interested in knowing the treatment effect in my study. I have data (mean, SD) for pre and post treatment.

01 March 2020 3,572 2 View

The pH differential method - how do i calculate the concentration of anthocyanin in a dried extract?

27 February 2020 9,573 5 View

How to begin slicing sections after a period in cryoperservation?

I have preserved large sections of sheep brain tissue in an ethylene glycol solution for later use. I'm hoping to take the sections out of solution and then slice on a microtome or cyrostat. How...

29 January 2020 1,884 3 View

Please i need an expert help on how to convert amino acids results expressed mg/l to residues??

Please what formula should I use to convert amino acid compsition results expressed in mg/l to residues? I ran a HPLC amino acid profile of a protein sample, the results came in mg/l but I need to...

18 January 2020 5,765 1 View

Is anyone aware of a study that compares the discourse around intra-European mobility and integration for Eastern and Western Europeans?

I am looking for a study that compares the discourse around intra-European mobility and integration for Eastern and Western Europeans. Mobility of Western Europeans is not addressed in public...

20 August 2019 3,036 2 View

How does one calculate the % metal extraction, distribution ratio for each pH value, log D and pH1/2 from log D vs. pH plot from AAS results?

21 May 2019 8,115 3 View

Does anyone know of some working antibodies against EEA1?

I am looking for a good antibody to label early endosome of mouse cortical neuron.

18 November 2018 3,875 2 View

Have you used Covidence for systematic reviews before?

I came across Covidence for systematic reviews, which is the tool of choice for Cochrane, and was wondering if actual users can share their experience with the tool. It looks like a pretty...

06 August 2018 8,846 11 View

What would cause squared multiple correlation to be greater than 1?

I am testing a SEM model and one of my endogenous variable is defined by two observed variables (GPA and ability). The problem is that the squared multiple correlation being returned on GPA is...

21 June 2018 5,335 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View