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Questions related to Biotechnological Techniques
I am running a reactor for the AD of agricultural waste, and unlike the BMP tests, I understand that the calculation of the biodegradability (efficiency of degradation) of the semi-continuous...
01 January 2020 6,672 3 View
Does anyone know about "plant milking" technology? If you have an article a book or a related text, please introduce me. Thanks a lot
04 April 2019 8,646 9 View
Colombian government intends to restart the spraying of coca crops with glyphosate formulations. As we know, there are plenty of negative effects that have been discovered on the ecosystems and...
03 March 2019 8,854 4 View
Greetings Does anyone happen to have a simple and clear yet complete Cpf1 knock-in/knock-out workflows regards
12 December 2017 10,503 0 View
we have a real time pcr applied biosystems can we use it as thermocycler only or cant ?
28 June 2017 6,315 3 View
Since Tobacco is tetraploid does it is necessary to design gRNA in such a way so that all allele of gene of interest is targeted and we will have perfectly silenced gene. If so how to do so.How to...
04 April 2017 5,538 1 View
I'm using pCP20 vector carrying gene for FLP recombinase to remove kanamycin resistance cassette from bacteria genome. I know that there are two version of this vector: temperature or L-arabinose...
14 January 2017 6,249 2 View
01 January 2017 5,057 2 View
In the original paper by Skehan et al., and the nature protocol by Vichai et al., they fixated their cells with TCA, without aspirating medium beforehand. In other papers (e.g. Papazisis et al....
01 January 2017 8,599 1 View
I'm having a problem with gelatin coating. My gelatin for some reason solidifies prematurely and when i look under the microscope, i can see a lot of lumps. I tried many different ways from...
20 December 2016 726 6 View
Cell clumps (possibly stringy/stickiness cell lysis and DNA) are observed. They are hard, if not impossible, to break up after centrifiguation. I am suspecting high confluence and maybe too long...
17 December 2016 7,368 1 View
There exists a dual growth characteristics for Rhodococcus opcaus PD630. Though at suspended growth, the OD is read easily I am finding rather difficult in reading OD during aggregated growth....
12 December 2016 5,804 2 View
12 December 2016 3,987 7 View
12 December 2016 4,482 1 View
Hello, It is a very general question. I am doing pigment extraction (phycocyanin and phycoerythrin) from cyanobcateria using liquid nitrogen freeze and thaw method. The method i am using does not...
12 December 2016 7,837 4 View
I am working on A431 cells and since few days the cell pellet after trypsinization and centrifugation is becoming purple instead of white. The cells in culture are looking healthy and are without...
12 December 2016 9,285 6 View
Can anyone help me suggesting a suitable test for identification of long chain polysacharide in my mushroom water extract. I have already performed Molisch's test, Fehling's test, Barfoed's test,...
12 December 2016 10,191 4 View
05 December 2016 9,943 3 View
I am working on E.coli fermentation process. For maintaining pH I am using ammonium hydroxide 20% solution. During the process ammonia consumption approx 50 ml/l. But I want to know how I...
15 November 2016 919 4 View
11 November 2016 3,490 5 View
I did not have answer from the company.
11 November 2016 8,514 0 View
For my current research, I need to find a method to measure fibrousness reproducable using TPA / viscometer...
11 November 2016 5,419 2 View
I am writing up the standard operating procedure for the autoclave and realised that there is a cycle called "Dissolution". I have not used it before but from the manual that I read, it is used...
11 October 2016 5,430 2 View
Hello, Can anyone, who is familiar with ADP-ribosylation, suggest me what is the best way to check if my protein is ADP-ribosylated? Thanks.
10 October 2016 818 4 View