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Questions related to Biotechnological Techniques
Hi, to determine the degree of hydrolysis of proteins,I want use the hoyle and merritt method. DH= 10% TCA soluble N in sample/Total N in sample *100 For the determination of total nitrogen in the...
05 May 2016 1,147 0 View
I am looking for malaria sporozoite freezing media which will give me viable sporozoite on thawing. The composition should preferably be not covered by a patent. The intended use is for basic...
05 May 2016 8,240 0 View
how to increase the metallic sheen of E.coli on EMB agar?
05 May 2016 498 4 View
Hi all, Currently, I working on a project to change the endotoxin test for purified water from gel clot to kinetic chromogenic. Previously, the PW sample tested by gel clot at 10X. I proposed to...
05 May 2016 7,843 1 View
I want to identify a particular protein from yeast. I tried to do MSMS. But it's never giving any hit with any protein from Saccharomyces cerevisiae. For your information I have only the SDS PAGE...
04 May 2016 1,661 5 View
In a culture cell system, I have some unexpected result with some cells after drug treatment (induce double strand break). I want to keep these cells in culture, but I want to be sure that the...
18 April 2016 3,437 2 View
I have an unidentified halophilic bacterial consortium that has been obtained through subculturing natural hyper saline polluted soil/water samples in sterile nutritive waste water with salt...
04 April 2016 8,045 4 View
I would like to store these samples for at least 6 months .
04 April 2016 9,775 3 View
What will happen if we innoculate five strains of Bacillus subtilis, B. cereus, B. amyloliquefaciens, B. pumilus and B. sphareicus together in a broth medium? Whether they all flourish well or...
04 April 2016 9,961 2 View
I'm trying to insert few point mutations close to each other in a gene of interest and I want to use CRISPR/Cas9. Clearly, I am aiming at HDR rather than NHEJ, so I will provide a template DNA...
25 March 2016 586 5 View
How would you make a solution or ointment (or otherwise) containing sucralfate where the sucralfate molecules are able to penetrate the skin?
21 March 2016 3,581 2 View
Hi everyone, I am using atecollagen from Koken company to make the cell beads for 3D cell culture. Intially, pH of the gel is 7.3-7.5, which is suitiable for gelation. However, After put these gel...
07 March 2016 6,797 6 View
Dear All, I want to perform KEGG enrichment analysis for some of my genes. Kindly suggest me any useful database or way to perform it. Thanks in anticipation.
03 March 2016 6,780 2 View
In human UGT1A1 seems to be the only relevant enzyme to glucuronidate unconjugated bilirubin into excreted forms (mono- and diglucuronides). Is the pathway the same for Cynomolgus monkey (Macaca...
03 March 2016 8,093 0 View
03 March 2016 7,868 6 View
03 March 2016 9,726 2 View
I made a dilution series of a mixure of different compounds and significant correlations (R2 > 0.99) between the response of the compounds and corresponding concentrations were observed. Does...
13 February 2016 9,927 3 View
OD > 0.300 Standard Deviation> 0.08 Negative control has high OD. Any advice I will listen!
08 February 2016 9,012 3 View
I am trying to store my slides carrying stained DNA using fluorescent dye (Hoechst DNA staining) for a long period of time. how can I do it?,...Do you have any advice?
07 February 2016 1,304 4 View
I will be glad if someone tell me the best pretreatment method that will do away with lignocellulose of orange waste, because we want to undertake a research on biogas production.
07 February 2016 8,407 3 View
Hello everyone Could you tell me how to make a simulated gastric and enteric medium exactly? with thanks
05 February 2016 3,583 1 View
We need dye calibration for our step-one real-time system. One of our friends have a calibration kit with single used. can we reuse it or not. Thank you for your attention. Mohsen
05 February 2016 8,365 2 View
02 February 2016 4,558 4 View
02 February 2016 7,223 4 View