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Questions related to Biotechnological Techniques
I thought UVB cannot reach to dermis, so using UVB rather than UVA for fibroblast is reasonable? And if you think so, what is the reason for it?
02 February 2016 4,892 0 View
are there any process to be applied on the oils? or if at all we can't prevent it, then what are the methods available to remove the thickened mass from the remaining oil and reuse the left over oil?
02 February 2016 7,331 1 View
OD > 0.300 Standard Deviation> 0.08 Negative control has high OD. Any advice I will listen!
02 February 2016 5,949 4 View
I am trying to store my slides carrying stained DNA using fluorescent dye (Hoechst DNA staining) for a long period of time. how can I do it?,...Do you have any advice?
02 February 2016 8,995 5 View
Hello everyone Could you tell me how to make a simulated gastric and enteric medium exactly? with thanks
02 February 2016 4,746 1 View
these days, I'm doing gene knockout mutation to use Red recombination including pKD119 (having araBAD promoter, exonuclease gene) and pKD3 (TetR) as helper and template (cassette) plasmid,...
02 February 2016 7,461 7 View
We need dye calibration for our step-one real-time system. One of our friends have a calibration kit with single used. can we reuse it or not. Thank you for your attention. Mohsen
02 February 2016 4,658 2 View
Direct Agrobacterium Transformation Of GV3101(PMP90RK) With PLH Vectors Thaw the competent cells carefully on ice (here it took 50 minutes). Add 0,1-1 µg of DNA to the cells and mix.Freeze the...
19 January 2016 1,123 2 View
The given IC-50 concentration in the data sheet of inhibitor 10 micro molars, to look at the inhibition of IL-8. The concentrations above the IC-50 seem to be giving considerable results for eg....
01 January 2016 5,678 0 View
Have you used expired DryStrips? Did they worked well?
12 December 2015 5,928 0 View
Hi, i want to know can we avoid use of LA taq polymerase because of not proofreading capability for ligation? Can we use another vector which is use for proofreading taq polymerase? Please suggest...
24 November 2015 9,210 4 View
11 November 2015 7,617 4 View
Since last few months I am trying to improve my 40kd protein crystals for which I am getting either needles or multinucleation. I have tried various conditions with varying pH, Temperature,...
11 November 2015 5,891 4 View
I am currently trying to separate different membrane compartments via differential centrifugation, isopycnic sedimentation and sucrose gradient, and I would like to visualize the membranes. I've...
11 November 2015 5,278 1 View
Hello everyone I am planning to do Bisulfite sequencing but my samples lacks the flanking region upstream and downstream for primer binding. So can anyone help me with possible solutions?
11 November 2015 2,536 3 View
I am designing a multiplex for 8 loci using 4 dyes, what should be the minimum distance between loci of the same dye colour.
11 November 2015 3,953 1 View
If I want to know the cell concentration in a sample (by absorbance), shouldn't I do first a separation by means of centrifugation to get rid of debris? in many protocols it is explained that I...
10 October 2015 8,724 19 View
Hi, I need to transfect a melanoma cell line with a plasmid but I’m not sure how much DNA (ug) is necessary for the transfection and which plate is better for this, 96, 24 or 6-well plate. I...
10 October 2015 7,611 3 View
Iam working on some bacterial strains and want to know whether they have glycine betaine or not.
09 September 2015 2,485 1 View
I would like to select a Streptococcus suis isolate, for further testing. Because of the possible flora, due to ingestion/inhalation, which out of these two organs could be considered more clean?
19 August 2015 8,378 3 View
08 August 2015 4,831 4 View
Can anyone please provide me CLSI document M27-A3 ? I want to perform broth microdilution method for antifungal susceptibilty testing but do not know how to do it..Can anyone please explain it...
07 July 2015 4,118 4 View
There is the specific interaction between target cells and therapeutic agents. I am focussing on the determination of possible cellular targets of therapeutic peptides. I think MS and RNAseq...
07 July 2015 5,333 2 View
Can I predict whether a protein is produced in a expression system (e.g. E. coli, mammalian) in advance?
07 July 2015 5,511 1 View