I am working on E.coli fermentation process. For maintaining pH I am using ammonium hydroxide 20% solution. During the process ammonia consumption approx 50 ml/l. But I want to know how I calculate the acetate formation during the process for which the ammonia used. E. coli fermentation is mixed acid fermentation so how I can calculate specifically acetate formation during the fermentation process.
Dear Rajendra Singh
you can do this by Follow-up acetate ....Good luck.
Dear Rajendra,
There is likely no correlation between acetate formation and ammonium hydroxyde consumption. Cell growth triggers base consumption as well: on minimal medium uptake of ammonium, incorporation of NH3 for amino acid biosynthesis and release of a proton. There is no way, but directly measuring acetate in the broth.
Best,
Christoph
I think you can assume this is an acetate-butyrate fermentation. We can assume the proportion of acetate:butyrate produced at the ratio 1mol:1mol (to be more accurate on this ratio, you can cross check by GC analysis first). You said that you need 50 ml of 20% ammonium hydroxide, means equivalent to 10 ml of pure (100%) NH4OH or assume to be equivalent to10 g NH4OH which means 10/35 mol NH4OH. Assume acetate require half (in mol) of that, i.e. here (5/35 = 1/7) mol which is 1/7 x 60 g = 8.6 g of acetatic acid approximately.
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