Hello,
It is a very general question. I am doing pigment extraction (phycocyanin and phycoerythrin) from cyanobcateria using liquid nitrogen freeze and thaw method. The method i am using does not specify how to thaw the samples, either room temperature or using water bath? Which one would be better? If anybody is familiar with this, pls give some suggestions.
Thank you!!!!
Dear Zoya,
I have worked on phycocyanin extraction for other organisms. Based on my experience, I did freeze and thaw by freezing at -80oC, continue with thawing at room temperature.
I did with -80oC to crystallized the cell wall which will be easier to break compare to use liquid nitrogen. It worked well with my extraction.
Good luck with your work
Best,
-Fean-
Yes, I tried to use liquid nitrogen. I found that freezing the sample in -80oC gave more products than in liquid nitrogen. For extraction, I only used buffer for the pigments.
Success with your work
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