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Questions related to Biotechnological Techniques
Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...
10 October 2014 7,140 9 View
Hi All, I have SK-N-SH cells I would like to differentiate, what protocol do you normally use? I can see that people do it in different ways, I want to know which method gives you the most...
10 October 2014 4,001 1 View
I have planned 14 days CIDR application in sheep. I will treat also eCG and PGF2 alfa 2 days prior to withdraw of CIDR. However, I do not exactly sure when I have to carry out Timed Artificial...
10 October 2014 8,885 5 View
Hi, I'm trying to transform E. coli DH10Bac cells with a pFB-Lic-Bse vector containing my gene of interest. 48 h after transformation, I get some white colony on LB-Km-Tet-Gent-Xgal-IPTG, but when...
10 October 2014 6,694 6 View
I am going to do induction in Pichia pastoris. But my lab does not have yeast nitrogen base. Just wondering if I can substitute with some other things.
10 October 2014 9,509 4 View
I need to recover randomly mutagenized plasmids and sequence for the mutation from each hit of a screen yielding ~100 hits. The two options I am considering are (1) a yeast plasmid isolation...
10 October 2014 8,425 1 View
Is there a method for the determination of saponins other than HPLC?
10 October 2014 9,116 2 View
I'm currently working on Lipase production from Bacillus subtilis. I could not find a detailed literature on components which are influencing the lipase gene expression. Can any one suggest the...
10 October 2014 7,189 11 View
These days some normal cells like fibroblast can also be transformed to desired cells type like neuron or cardiac myocytes or blood cell progenitors or other cells instead of inducing pluripotent...
10 October 2014 9,468 6 View
I am trying to Electroporation of Synechosystis cells using 2.5 Kv ( 12.5 KV/ Cm) voltage in 2mM, 1 mM HEPES (pH 7.5)or 5 mM NaCl. I washed the cells three times before set for Electroporation...
10 October 2014 8,942 9 View
Hello, is there anybody currently working on cell-secreted protease which is mainly focused on Matrix Metalloproteinase 9? I'm new to cell manipulation research. I'd like to ask whether we should...
10 October 2014 9,545 3 View
I want to test our siRNA effects in vivo, wondering which delivery method would be the best, either locally or systemically.
10 October 2014 977 4 View
If I integrated M13-SSR (5ʹ-CACGACGTTGTAAAACGAC-3ʹ) within the forward primers of the Ym30 pair primers which is F: CACGACGTTGTAAAAC R: CACGTATTAACTCCAT . Can I write the new integration of the...
10 October 2014 5,513 1 View
Considering a phenotypic assay (proliferation/viability), would you transfect together with the target gene siRNA also negative ctrl siRNAs to achieve the same concentration as the multiple target...
08 October 2014 6,338 3 View
I am isolating okazaki fragments and want to prepare sequencing library from them. To this end I need to get rid of all the RNA nucleotides linked to the DNA (the RNA primer of the Okazaki...
06 October 2014 3,342 7 View
I would like to design promoters against porcine IFNs. Any suggestions?
03 October 2014 2,027 2 View
01 October 2014 616 2 View
01 October 2014 6,724 5 View
In Platinum-E cells , by co-transfecting pBabe retroviral transfer vector and pVSVG vector, can retrovirus be generated. We have been successful with mscv retroviral backbone plus VSV-G vector,...
29 September 2014 5,134 2 View
Cas9 is a good genome editing tool. I want to make stable cell lines using CAS9 knocking in tech. I don't know how to choose hot spot for inserting. Does anybody know about it?
26 September 2014 1,596 2 View
Please suggest to me how I can calculate N/P ratio during delivery of a siRNA (25 oligonucleotides) using branched PEI (mw 50000).
26 September 2014 4,217 1 View
Within my project I want to use CRSIPR Cas9 to generate stable knock-in cell lines. The goal is to knock in a gene with a point mutation which leads to a single amino acid substitution. I read a...
24 September 2014 1,514 5 View
I'm designing an experiment to see if RNA is bound to proteins in my sample. Can I use RNase and Proteinase K together, or will the protease break down the RNase?
24 September 2014 1,439 2 View
I'm currently using the RiboMax T7 transcription kit from Promega and I am able to get transcripts from my DNA template of 1.6 kb but am having difficulty getting the longer transcripts. I am...
21 September 2014 3,230 2 View