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Questions related to Biotechnological Engineering
I want to know all the unique characteristics of Archaea, that made them able to survive any type of environmental condition.
31 December 2019 7,311 4 View
I wonder something abouth processing and making biosimilar. I understood if a biotecnological durg patent is done and if we make similar like it, its become biosimilar, is't it? So, if I took a...
31 December 2019 8,747 2 View
Hi, I was wondering if commonly used tagging systems (like FLAG, Streptag or His) can be used as a docking site for knocking down -or even knocking out- tagged proteins The goal would be to add...
11 December 2019 1,147 3 View
How we can identify and isolate archaeal strain from the invironment?
11 December 2019 8,339 4 View
When we have a positive regulation the control is tight, so we have low background expression under non-induced conditions. Negative regulation can not be fully controlled, but as far as I...
25 January 2019 2,032 5 View
why is MG1655 used to conjugal transfer using as donor strain WM3064 ? this mechanism is used in article= Toward Bioremediation of Methylmercury Using Silica Encapsulated Escherichia coli...
18 November 2017 1,862 2 View
mention the instrument and methods used to measure the surface roughness of the sand and sand submerged in the water i.e wet sand.
01 September 2017 4,874 4 View
Are traditional methods " selection and crossbreeding" peter or use MAS, or we must combine between traditional and modern methods. Can I create a new chicken breed? Are create a new chicken...
08 June 2017 2,151 3 View
I quantify the amount at the begining and after the addition of fungus of the genus Aspergillus, but I don´t know why is there a decline in the amount. The results are in the image. Thanks if...
05 June 2016 2,761 2 View
Is GSH assay is the best method to find the oxidative stress in silver and gold nanoparticles ? If not which method is suitable to find ROS ?
26 May 2016 956 2 View
HI, I need some advice for growing stem cells in carbon nanotubes,
22 May 2016 5,215 3 View
Can anybody tell me the procedure to synthesize N,N- Diethyl - 4- Nitroaniline from 4- nitroaniline ?
06 January 2016 4,771 1 View
I am after some hydrogels where the stiffness of the matrix can be tuned, the hydrogel needs to be biocompatible as it will be used for cell culture with neural cells.
24 November 2015 4,180 6 View
Hi, If anybody can help me with the sequence of pGPTV-BAR vector. I need the sequence of BAR gene or Bialaphos gene. I couldn't find the sequence on ADDGENE. Please help me!!!
25 July 2015 1,285 3 View
Nowadays, lignocellulosic composition can be determined by using thermogavimetry analysis. However, there is some limitation on it, for example lignin information cannot be obtained. On the other...
01 June 2015 1,645 3 View
Metal oxide nanoparticles in water or nutrient solution produce a small amount of metal ions. Can plants take in MNPs within the system and then convert it into metal ions, increasing the amount...
09 March 2015 9,489 3 View
I need to adapt my anaerobic microorganisms to feed on dye instead of sucrose for carbon. The dye I'm using is an acid red 14 type. Thanks
02 March 2015 5,278 3 View
I'm designing a bioprocess using Nicotiana Tabacum BY-2 plant cells, I need it's kinetic parameters, thank you.
23 February 2015 8,168 11 View
I am facing problem in filter integrity during sterilization with fermenter. Every time after sterilization, integrity test of filter has failed. I am using PTFE membrane filter which are...
29 January 2015 6,864 3 View
Please refer to me some Bio-tech company websites to purchase this kind of stuff?
30 September 2014 7,860 3 View
I am currently researching a membrane-based method for virus purification, and I would like to compare it in economic terms to Density gradient centrifugation (DGC) in industrial scale. Which...
22 September 2014 7,922 2 View
I am currently trying to generate stable cell lines using pSpCas9(BB)-2A-Puro (Addgene plasmid ID: 48139). The sgRNAs that were designed are successful in knocking down the protein of interest,...
21 April 2014 1,817 12 View
Natural carbon source.
19 March 2014 3,305 4 View
I would like to determinate the production of this enzyme by colorimetric assay
06 February 2014 636 9 View