Since Tobacco is tetraploid does it is necessary to design gRNA in such a way so that all allele of gene of interest is targeted and we will have perfectly silenced gene.
If so how to do so.How to find out the sequence of each allele of the gene of interest.
Md Hassanur Rahman
Hello Lohani !
Following article will be helpful for your research
CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum.
http://link.springer.com/article/10.1007%2Fs11103-014-0263-0
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