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Questions related to Biotechnological Techniques
Specifically epinephrine and norepinephrine in human plasma
09 September 2014 8,054 8 View
I have transformed rice plant by Agrobacterium mediated gene transfer system but getting problem of contamination by fungus. Is there any problem if I use antifungal in that plate to get rid of...
09 September 2014 10,186 3 View
Hello, I was wondering if anybody is aware of a method I could use in mice to blunt PKA and/or MAPK signaling in adipose tissue. I have tried i.p. administration of H-89, which did not have any...
09 September 2014 5,118 6 View
I'm currently using the RiboMax T7 transcription kit from Promega and I am able to get transcripts from my DNA template of 1.6 kb but am having difficulty getting the longer transcripts. I am...
09 September 2014 2,025 2 View
I am doing in situ hybridization searching for mRNA on paraffin-embedded tissue of dogs.
09 September 2014 2,060 8 View
Please suggest to me how I can calculate N/P ratio during delivery of a siRNA (25 oligonucleotides) using branched PEI (mw 50000).
09 September 2014 6,098 1 View
Cas9 is a good genome editing tool. I want to make stable cell lines using CAS9 knocking in tech. I don't know how to choose hot spot for inserting. Does anybody know about it?
09 September 2014 7,381 1 View
I'm designing an experiment to see if RNA is bound to proteins in my sample. Can I use RNase and Proteinase K together, or will the protease break down the RNase?
09 September 2014 6,322 2 View
Within my project I want to use CRSIPR Cas9 to generate stable knock-in cell lines. The goal is to knock in a gene with a point mutation which leads to a single amino acid substitution. I read a...
09 September 2014 7,416 6 View
Hi! I´m trying to analyze results from a competition binding assay to assess the possible heterodimerization of GPCRs. As far as I know, the best way to fit the data is using the two-state dimer...
09 September 2014 7,183 4 View
In Platinum-E cells , by co-transfecting pBabe retroviral transfer vector and pVSVG vector, can retrovirus be generated. We have been successful with mscv retroviral backbone plus VSV-G vector,...
09 September 2014 3,764 2 View
Before, my PCR band is of 600bp but after no bands appear, I am using MspI enzyme...........
09 September 2014 3,234 15 View
I consider buying a MiVac Duo with the coiled SpeedTrap as an alternative to Savant/metal cold trap for drying/pre-concentrating LC fractions from tissue extracts. My main attraction is their...
29 August 2014 8,841 2 View
Without any other regulation in Cell. We know that ethanol as disinfectant to microbial cell.
28 August 2014 6,402 8 View
08 August 2014 524 2 View
08 August 2014 10,044 9 View
I am performing enzyme assay of Xylitol Dehydrogenase and Xylose reductase but I don't have any reference value for that. So tell me what satisfactory value of activity form ADH and XR can be...
08 August 2014 3,735 5 View
Focused to recombinant protein expression.
09 July 2014 2,942 2 View
07 July 2014 1,727 2 View
Which solvent is better for extraction curcumin by microwave method?
07 July 2014 6,432 2 View
I have been trying to develop a TLC system for an unknown compound (biosurfactant), but there are so many spots and trails in TLC and no solvent and their ratios have been proven appropriate for...
23 June 2014 6,820 11 View
When I prepare TLC plates with only water, then it works well otherwise not. I want to know about the solvents which are also helpful when we have to use spraying reagents. Can anyone please help?
06 June 2014 9,578 4 View
I am going to optimize the medium composition for enzyme production. there are many optimisation method such as RSM, Taguchi Orthogonal array etc... Which method is best for medium optimisation?
08 May 2014 2,809 11 View
05 May 2014 1,726 12 View