20 December 2016 6 434 Report

I'm having a problem with gelatin coating. My gelatin for some reason solidifies prematurely and when i look under the microscope, i can see a lot of lumps. I tried many different ways from leaving longer in the warm bath, to vortex in between pipetting but none worked. I don't know what else can I do to be honest. 

After pipetting the gelatin onto the coverslip, I leave them at room temp. (side a box to prevent bleaching) for 15 mins, and suck out the excess. Do you think any of these steps make it solidify unevenly? 

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