I am working on E.coli fermentation process. For maintaining pH I am using ammonium hydroxide 20% solution. During the process ammonia consumption approx 50 ml/l. But I want to know how I calculate the acetate formation during the process for which the ammonia used. E. coli fermentation is mixed acid fermentation so how I can calculate specifically acetate formation during the fermentation process.
Dear Rajendra Singh
you can do this by Follow-up acetate ....Good luck.
Dear Rajendra,
There is likely no correlation between acetate formation and ammonium hydroxyde consumption. Cell growth triggers base consumption as well: on minimal medium uptake of ammonium, incorporation of NH3 for amino acid biosynthesis and release of a proton. There is no way, but directly measuring acetate in the broth.
Best,
Christoph
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