Can anyone help me suggesting a suitable test for identification of long chain polysacharide in my mushroom water extract. I have already performed Molisch's test, Fehling's test, Barfoed's test, Iodine test which are tests for qualitative analysis of Carbohydrates. But I got negetive result in all tests.
Hello
Mr Prabhat refer this paper to get information.
Quantification and identification
of polysaccharide contents in
Hericium erinaceus
This is not an easy question. Two methods that can be tried are DIONEX chromatography of an acid hydrolyzate (2M TFA 121C 2 hours) using standards for reference or methylation analysis (permethylation of polysaccharides, methanolysis/hydrolysis, reduction with NaBH4 or NaBD4, acetylation and analysis by GC-FID and GC-MS).
Hello!
LC-ESI-QTOF-MS may be an auxiliary method for identification of long chain polysaccharide in your mushroom water extract. How to identify them by LC-ESI-QTOF-MS, you can refer to the below articles.
Jiang Y, Zhong M, Peng W. Qualitative analysis of plant-derived samples by liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry. Tropical Journal of Pharmaceutical Research, 2015, 14(5): 925-930.
McGhie TK, Hudault S, Lunken RCM, et al. Apple peels, from seven cultivars, have lipase-inhibitory activity and contain numerous ursenoic acids as identified by LC-ESI-QTOF-HRMS. Journal of agricultural and food chemistry, 2011, 60(1): 482-491.
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