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Questions related to Biotechnological Techniques
Can anyone point me to some useful references on methods to assist in clarification of crude homogenates of animal tissue? Centrifugation in our case is only partly successful and our current...
29 April 2014 2,947 1 View
I have decided to give a concortium of probiotic to marine fish, but the problem is that dont know the resistance of this probiotic. Perhaps there are any suggestions or ideas about a fast way to...
21 April 2014 8,645 3 View
04 April 2014 2,228 3 View
Date palm micropropagation
04 April 2014 5,373 0 View
In this study we evaluated the optimal physical requirements such as the shaking rate and incubation time for applying in liquid media malt extract broth to obtain a high emestrin compound and its...
04 April 2014 7,554 4 View
Filamentous fungi electroporation.
12 March 2014 4,224 1 View
I am trying to express hGM-CSF in S. cerevisiae. I use strain BY4741 and vector p427-TEF/MF-hGM-CSF. I've already gotten many recombinant clones (checked genotype) but I can't get secreted...
08 March 2014 2,091 16 View
What are the techniques used?
03 March 2014 8,119 0 View
Cellulolytic microorganisms are necessary to get a good biocomposting fertilizer. However, the methods to count these organisms depend on type of cellulose in the culture media. We have been using...
03 March 2014 2,236 0 View
I am trying to find about the in vitro development of fungal contamination in particular when they are exposed to carbon sources present in the culture media.
03 March 2014 6,111 0 View
To use lactobacillus as probiotics.
03 March 2014 1,911 8 View
03 March 2014 3,754 1 View
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02 February 2014 4,215 1 View
I want detailed examples
02 February 2014 3,437 4 View
What would be the key points to be addressed while expressing a membrane protein from gram negative(E.coli) in gram positive clostridium acetobutylicum?
02 February 2014 2,180 0 View
The catalysis of organic compounds by whole cell fungi takes a lot of time, 10-20 days. Is there some method to do the reaction faster? Sometimes, the enzyme does not work for specific compounds....
02 February 2014 1,580 1 View
I'm curious whether anyone on here has experience with InvivoGen's PlasmoTest to detect Mycoplasma infection of cell culture lines, as well as perhaps how it compares with PCR and Hoechst...
14 January 2014 853 8 View
I need to standardize the growth phases of yeast cells as quick as possible. However, with the plate count and haemocytometer method due to the large no. of isolates. So, can anyone provide me...
01 January 2014 8,618 8 View
We are conducting a design project where the production of Amevive is our topic. We realise that it was subsequently taken off the market due to it's ill effects on the patient. However, any...
01 January 2014 6,996 3 View
I want a simplified method of using acetone, ethanol and methanol for the extraction process, the measurements of the algae against the solvents and also how to determine the stability and index...
01 January 2014 5,204 0 View
I have to make a growth curve but I have too many samples to be considered simultaneously. Is there any other way of monitoring the growth instead of measuring the optical density every 30...
13 December 2013 842 2 View
I have recently got some problem with my 2-D gels. Please check the attached gel pics for details. I have used 2-D clean-up kit (Biorad) to remove the impurities from the sample. I think there...
12 December 2013 6,557 8 View
I'm looking for available options for chromogenic substrates for pectinases; I would need, for example, 4-Nitrophenyl-Alpha-D-Galacturonide, but this substrate seems hard to find . Sigma-Aldrich...
12 December 2013 3,274 4 View
I'm working with the plant physalis peruviana, I'm doing a extraction with the roots of this plants, and in this extracts I'm measuring the antioxidant activity. I'm using a spectrophotometric...
08 December 2013 8,527 29 View