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Questions related to Biotechnological Techniques
I am writing up the standard operating procedure for the autoclave and realised that there is a cycle called "Dissolution". I have not used it before but from the manual that I read, it is used...
10 October 2016 9,217 2 View
Hello all, I have done my masters in Italy. Now i am in part of diabetic research dealing molecular biotechnology techniques and Histology based techniques. I am really interested to join PhD in...
10 October 2016 5,964 0 View
I'm looking to isolate Hematopoietic Stem Cells from fetal liver tissues. Currently the only markers that have been used to identify potential HSCs are CD34 and CD45. Can anyone suggest some...
10 October 2016 944 1 View
The solid medium used for Legionella app is BCYE, which has charcoal inside. Looking up the literature I only found it was first used to replace starch in the medium. But still couldn't get the...
10 October 2016 6,183 10 View
07 October 2016 8,083 1 View
Hi, I would like to modify the surface of carboxylic acid functionalized CdTe Qds with thiolated oligonucleotides. Can anyone help me for protocol to create thiolester bond between (CdTe...
05 October 2016 4,171 1 View
I have 2 questions concerning this issue: 1- When to use KCl saturated solution and when to use KCl saturated with Ag+ and is it a big deal in case I used them in exchange 2-Different electrodes...
09 September 2016 8,418 1 View
I was working with luciferase assay to see the ability of my compound as a ligand for receptor. The result somehow does not follow dose dependent manner. it reachs optimum fold change at low...
12 August 2016 4,016 3 View
Hi, I am trying to grow N.gonnorhea on chocolate agar plates and I dont seem to be getting lawns after 24 hrs of incubation at 37 degree Celsius and 5% CO2. I do not get it where I am going wrong
08 August 2016 776 1 View
08 August 2016 3,309 3 View
03 August 2016 1,423 1 View
Is there any difference in performing ChIP on suspension cells vs. adherent cells? I'm currently using Activemotif ChIP-IT Express Enzymatic Kit
26 July 2016 8,419 3 View
Two identical point mutations occured after sequencing of two of my colonies (DH5alphas+pGEMTeasy with insert).To amplify the region of interest I have performed a genomic PCR with Pfu polymerase,...
25 July 2016 10,015 6 View
I am currently working with cellulose nanoparticles. I would like to test if the polymer I am using are adsorbing or not onto their surface. There are different techniques such as Isothermal...
20 July 2016 1,108 3 View
Hi. I am using a malachite green assay to measure phosphate production for ATPase activity (MAK113 Sigma ATPase/ GTPase Activity Assay). In the protocol it says to subtract the absorbance...
07 July 2016 2,280 5 View
Hi everyone.. A brief intro first I am trying to do one assay with my peptide conjugated with GFP and the control is GFP. I purified both proteins using Ni-NTA as they possess His-TAG. The purity...
07 July 2016 3,902 2 View
07 July 2016 8,953 2 View
06 July 2016 6,713 4 View
Protein is a homodimer. Which approach would be more appropriate?
05 July 2016 638 1 View
In your opinion, what is the best flow cytometer that currently exists in the market to analyze microparticles? Thank you
16 June 2016 4,850 3 View
In a fermentation process it was found that residual fungicides that were sprayed on ginger root negatively affect yeast growth and performance. We have resorted to a time consuming method of...
06 June 2016 1,323 2 View
Specically i want transform Nicotiana tabacum . Thanks
17 May 2016 8,367 3 View
Hi all, Currently, I working on a project to change the endotoxin test for purified water from gel clot to kinetic chromogenic. Previously, the PW sample tested by gel clot at 10X. I proposed to...
13 May 2016 6,200 1 View
how to increase the metallic sheen of E.coli on EMB agar?
10 May 2016 3,783 4 View