Greetings
Does anyone happen to have a simple and clear yet complete Cpf1 knock-in/knock-out workflows
regards
What are your thoughts about food produced from organisms that were genetically edited using CRISPR tool box? To be more precise, is there any one from the Anti-GMO community who thinks that...
11 December 2017 4,531 26 View
I once heard a phrase that says "We're now training a generation will be hired in jobs that we don't know yet" (very catchy!) Can this phrase be acceptable in the field of agricultural experts...
11 December 2017 2,414 11 View
A- Fruit sweetness level – from 1 to 10 what do you prefer when 1 is the sweetness of avocados and 10 is the sweetness of mangoes B- What would you chose to consume from the following 1- You...
11 December 2017 4,972 14 View
Welcome to E-NAMTILA, the amazing source of the Land's radiance, the place where the destiny of all the lands is decreed and the living people are correctly guided (ETCSL translation:...
01 January 1970 9,696 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View