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01 January 2015 5,924 5 View
I may not know enough to know if this is even possible, but if it were: who, what industry, where would be likely to be working on something like this? I am sure there would be more interest in...
12 December 2014 9,654 4 View
I understand every protein is different regarding induction and solubility. I'm just wondering if there is a rule of thumb regarding a molecular weight limit. I'm thinking about trying to express...
12 December 2014 1,569 7 View
I am doing the HLA-typing by NGS before starting the same I need to understand what the HLA-typing is and about the nomenclature aspects. I have not found any articles which give me complete...
13 November 2014 3,482 3 View
I took the entire sequence that exists between the one gene and the next gene (which is to be expressed) as the promoter sequence. The region has three probable promoter regions.
11 November 2014 476 10 View
Hi all How can to regulate stability and half-life of RNA In cell ? and what is its effect on gene expression?
11 November 2014 1,808 0 View
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11 November 2014 6,969 0 View
I want to clone a gene in Pgemt vector having BamH1 frw enzyme and Sal1 frw enzyme. where as in Pgemt vector BamH1 is considered to be a enzyme which "Restriction Enzymes That Do Not Cut the...
11 November 2014 1,984 3 View
Am doing an inverse PCR, and totally stuck in the digestion step. None of the enzymes I used seems to work at all ( BClI, MfeI or EcoRI). I get a smear with the dense part only on the top, have...
11 November 2014 9,207 6 View
11 November 2014 7,973 3 View
We precipitated the proteins using methanol-chloroform protocol and redissolved the obtained protein pellet in Tris/PBS buffers. But the proteins are not completely dissolved leaving behind a mass...
11 November 2014 4,090 4 View
I’m trying to assemble 4 fragments (2.1kb vector + 3 inserts, each around 1.1kb) using Gibson assembly. All three inserts have the same sequence at the beginning and at the end (200bp promoter and...
11 November 2014 5,158 5 View
06 November 2014 6,152 4 View
05 November 2014 325 4 View
I'm currently working on Lipase production from Bacillus subtilis. I could not find a detailed literature on components which are influencing the lipase gene expression. Can any one suggest the...
26 October 2014 426 10 View
Hello, is there anybody currently working on cell-secreted protease which is mainly focused on Matrix Metalloproteinase 9? I'm new to cell manipulation research. I'd like to ask whether we should...
20 October 2014 4,691 3 View
I am having 2 issues with this antibodies (primary antibody from Santa Cruz Biotech): First: The aliquots that I prepare are evaporating. Second: They are not lasting enough to compare them with...
15 October 2014 2,736 7 View
These days some normal cells like fibroblast can also be transformed to desired cells type like neuron or cardiac myocytes or blood cell progenitors or other cells instead of inducing pluripotent...
14 October 2014 581 5 View
Hi all,May I know what is the role of acids (HCl, H2SO4, Acetic acid, phosphoric acid, Oxalic acid and nitric acid) in removing lignin/ cellulose/ hemicellulose in lignocellulosic biomass?If we...
13 October 2014 6,059 10 View
I'm trying to insert a 120bp segment into a 4k vector using Gibson Assembly. The purpose is to replace a 27bp portion of the vector with NNKs. The insert is ordered as an ssDNA oligo, and then...
10 October 2014 9,515 3 View
How can this be calculated? Is there any way to calculate by using matlab program?
10 October 2014 3,382 0 View
Considering a phenotypic assay (proliferation/viability), would you transfect together with the target gene siRNA also negative ctrl siRNAs to achieve the same concentration as the multiple target...
10 October 2014 4,007 4 View
We used a endotoxin free kit to prepare our plasmids. But we found IFNα proteins could not trigger IFN-dependent reporter genes in A549 cells when compared to mock treatment. We therefore...
10 October 2014 2,264 9 View
I would like to design promoters against porcine IFNs. Any suggestions?
10 October 2014 8,970 2 View