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Questions related from Hafiz Muhammad Rizwan
Dear Researchers, I am studying genome-wide identification and expression analysis of a gene family in passion fruit, which has a role in biotic and abiotic stresses, In previous reports mostly...
18 August 2021 7,741 7 View
Dear researcher, After amplifying my target gene (1900bp) into the required cDNA by PCR,>cut specific gel bands and purify the gel and the concentration was 30ng/ul. Next, performed A-Tailing...
22 January 2021 4,101 3 View
Dear researchers: If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression...
21 January 2021 5,741 2 View
Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...
16 January 2021 2,489 3 View
Dear Researchers, While working on plant gene transformation, so after cDNA + Gene Primer amplification, >the PCR/Gel were purified, so next for A-tailing and TA cloning is mandatory to...
13 January 2021 3,489 34 View
Dear Researchers, I am using pCAMBIA1301/1302 vectors for overexpression study, so can use the same vectors for transient expression? or need to design another vector for transient expression;...
09 January 2021 8,148 2 View
Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...
07 January 2021 8,660 99 View
I have identified fungus isolates from the fruit surface by SSU +ITS+ LSU regions, such as : 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus...
16 July 2020 5,735 2 View
I have different fungus isolates such as; 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus ; 5) Aspergillus flavus ; 6) Penicillium...
02 July 2020 7,001 3 View
I need a list of an internal standard that can be used for GC-MS based on fruit cuticle waxy compounds. Thank you in advance
21 May 2020 6,238 0 View
Dear Researchers: I want to do an experiment about the cuticle wax chemical composition and concentration of fruit surface at the different developmental stage so I would like to ask some...
21 May 2020 7,135 0 View
Dear Researchers, I am working on the gene expression of Passion fruit wax biosynthesis. I have selected the sequence of the primers (forward and reverse) from a published article about Passion...
05 May 2020 3,217 3 View
What are the parameters for selecting transcription factors bound to the promoter region of fruit cuticle wax biosynthesis?
03 May 2020 2,547 0 View
I already have the sequences of a DNA, (PCR product), sequenced from the forward and reverse primers, so how can i generate a contig sequence by using the Bioedit ?
29 April 2020 9,381 4 View
I want to extract the fungus isolate DNA for Sequencing and Identification as i have different isolates from fruit peel, so i want to know that is there some requirements for colony grow...
13 January 2020 8,223 3 View
I have isolated about 20 different kinds of Fungals from the fruit peel and now I would like to measure the spore concentration, 1- How can prepare the spore suspension solution for spore...
06 January 2020 2,766 15 View
i am working on two fruit varieties and their differemt gene expression profile such as genes in volves in fruit cuticle wax biosynthesis. as according to references i have found some KCS,...
06 January 2020 4,375 6 View
Dear Researchers, 1- I am working on RNA seq, and now I have transcriptome data and I have the different genes of interest from different resources, but I am looking for their full-length CDs...
02 January 2020 9,491 4 View
which one is the best microscopy among SEM. TEM. LEM .. for fruit peel cuticle wax thichness measurement? and how can make sure the cuticle wax layer ?,
27 December 2019 4,673 11 View
i am working on fruit cuticle wax composition at harvesting stage through GC MS,and want to measure cuticle waxy layer thickness through SEM for comparison in two varieties. (Scanning...
27 December 2019 5,031 7 View
Dear Researchers, I did Transcriptome of my two Fruit varieties at developmental, ripening and harvesting stage for the comparison of their differential gene expression profile, such as Sugar...
24 December 2019 6,674 4 View
i have isolated different fungi from fruit and now i have different fungal colonies, but i would like to identify that different fungus. Please suggest me the best method for preparation of slide...
15 November 2019 1,514 4 View
I have different fungal colonies so now i would like to identify them, so Which microscope is suitable and easy to operate for identification and taking pictures of fungus?
15 November 2019 9,750 3 View
Dear Researchers, I did the qPCR, 20ul, (10 ul TB gree, primer 0.4, template 2ul, Water 7.2), steps, by using TAKARA, I got the following results, as you can see that with 1X, 3X, and 5X...
29 September 2019 4,354 9 View
Dear Researcher I am working on passion fruit peel at ripening stages, and want to do qPCR but facing problem in getting good quality RNA from fruit peel, I have tried to extract the RNA...
28 September 2019 1,907 3 View
I have extracted RNA by usung Trizol method from fruit peel, and during nanodrop biospectrometer facing this error as in image. so need your suggestion . thanks
15 September 2019 775 4 View
i have RNA Seq Transcriptome data from company, but now i dont know how to analysis step by step.. can someoen share link about step by step guidence to analysis the transcriptomic data .?...
15 September 2019 1,485 11 View
i have RNA transcriptomic data and i would like to select a pathway for further analysis, but in transcriptomic ,have many pathways, so on which basis, and how to select a specific...
15 September 2019 4,100 3 View
Dear Researchers, I did the Gas chromatography mass spectrometery ( GC-MS) by using Agilent instrument, and now the Agilent software is not working in my computer, so any other easily...
07 September 2019 6,484 16 View
dear researcher, i have extracted RNA having 400-600 ng/ul concentration, A260/A280 is 1.98-2.00, but when i run gel there is no clear bands, so i have tried different ways to get...
05 September 2019 621 1 View
Dear Researchers, I have extracted RNA from passion fruit peel from fruit setting to ripening stages, (1- 10 developmental stages), by using Qiagen Mini plant Kit, from 1-5 stages the...
31 August 2019 1,874 0 View
Dear Researchers, I did scanning electron microscopy (SEM) of the fruit peel and founded different structure during SEM, Are these peel cells (box like) or epicuticle wax cells/layers?
21 August 2019 8,601 0 View
Dear Researcher, I would like you to help me in the answer to the following questions about passion fruit; as I have tried on google but I have not found latest and authentic reference about...
21 August 2019 3,649 1 View
Dear Researchers, I did scanning electron microscopy (SEM) of the fruit peel and founded different structure during SEM, Are these fungus body structure (mycelium or hphae) or any other things?
21 August 2019 4,275 0 View
Dear Researchers, The following pictures are taken under scanning electron microscopy (SEM) by using the fruit peel at harvesting stage, in picture can see the whitish rode shape structure...
21 August 2019 4,397 0 View
Dear Resrearchers, I am doing Gas Chrometography data analysis by using Aligent Qualitative Analysis B.07.00 and NIST 2.00 for cuticle wax compounds identification, (biochemistry) I would like...
18 July 2019 9,278 4 View
Dear Researchers, we take yellow passion fruit samples ( sweet and sour variety ),for RNA extraction from pulp and peel , and also was want to do physicochemical analysis, ( our main concern was...
16 July 2019 9,053 0 View
Dear Researchers, I am doing Gas Chromatography by using n-tetracosane as internal standard for un-targeted compounds , i am facing the following problem: 1: I run n-tetracosane as internal...
30 June 2019 7,758 10 View
Dear Researchers, I would like to do RNA analysis from passion fruit and want to extract RNA from passion fruit pulp but the problem is that passion fruit pulp quantity is very low and is...
13 June 2019 8,675 3 View
Dear researchers, i would like to make a research on "high quality organic fertilizer production from biogas extracts" looking forward to your sugessions
09 June 2019 9,546 14 View
Dear Researchers, I did Scanning electron microscopy and have pictures, but now i would like to measure the size of Stomata and trichomes on waxy layer, How can i measure the size?
26 April 2019 3,167 6 View
Dear researchers, i am working on fruit cuticle wax and i did GC-MS and have raw data of different chemical compounds from which some compounds have very long name, which i have not find from...
25 April 2019 1,411 6 View
I had done GC/MS for fruit peel for the identification of cuticle wax compound in fruit peel, now i have data in excel form but i want you to guide me that how to treat data generated by...
23 April 2019 7,792 3 View
Hello Researcher, I am working on Passion fruit and i need a protocol for sample preparation for sugar acids in passion fruit juice by HPLC, waiting for your kind suggestions and references
05 January 2019 8,277 9 View
Hello Everyone, extracted the fruit juice from a fresh harvested ripened fruit bu due to the shortage of time we kept our fruit sample in refrigerator for further study ( pH, sugar, acidic...
07 December 2018 7,601 8 View
Hello every one If we have three kind of fruit samples such as Fruit Pulp, Fruit Peel, and Fruit Juice. which sample will be best to extract RNA for further Genetic study ? Can use...
07 December 2018 4,911 13 View
I am working on Okra, during my research work i have experienced that after inoculation of hypocotyl and cotyledonary explants into full MS medium supplemented with different PGRs, callusing...
21 February 2018 8,675 1 View
Hello everyone Is there anyone doing research on the 1: In vitro effect of nutrient medium ( MS medium) on callus/ shoot induction? 2: How nitrogen affects the shoot induction in vitro ? 3; Is...
17 February 2018 7,987 2 View
