Dear researchers:
If the plant genome has not been released yet, can Unigenes from RNA sequences be used after BLAST and confirmed with known Arabidopsis genes for function/overexpression studies?
If, just in case, the plant's genome will publish during that experiment, would it have an impact on the experiment?
TheUnigenes/protein fron RNA sequence will be same in length and function in the genome as in the RNA sequnence Or will it be different?
Hafiz Muhammad Rizwan < If the plant genome has not been released yet ...>
Were you talking about your research plant species, Passion Fruit (know this from your other thread)? Their genome has not sequenced?
Yuan-Yeu Yau
Yes; about Passion fruit,
As the genomes on passion fruit published this year but started work on passion fruit 2 years ago by RNA-Seq. At that time no genome reference, so selected the target genes (CDS), for example, XYZ on the basis of Arabidopsis and RNA-Seq, and constructed the binary vector with target genes.
When compared those target genes (CDS) with genome and found that some gene has more than 90 % identity RNA-Seq and Genome, but some genes < 40%, so will its influences while publishing?
Or should re-assemble the RNA-Seq with the passion fruit genome? (as before RNA-Seq has higher similarity with Populus Trichocarpa")
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA