Dear Researchers,

I am studying genome-wide identification and expression analysis of a gene family in passion fruit, which has a role in biotic and abiotic stresses, In previous reports mostly researchers used seedling to apply stresses for the analysis of the expression levels, but I was considering using the passion fruit peel or pulp as material after biotic and abiotic stresses for qPCR expressions,

1- which material will be recommended, Peel or Pulp or both?

2- For biotic stress, I considered applying different pathogenic fungi on peel and will collect the peel samples after rotten occurs (5-7 dpi), and control samples will be without fungus infection.

So that will be fine?

2- And for abiotic stresses which parameters are more suitable - low or high temperature, or should be both? according to reports, the storage temperature of passion fruit is 3-5 C, so for abiotic stress should be used temperatures? For example; 0, 2, 4, 6, 8 C, and 4 C will be considered as control, so is this all right?

Your suggestions will be welcome

Regard

Rizwan

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