Dear Researchers,
I am studying genome-wide identification and expression analysis of a gene family in passion fruit, which has a role in biotic and abiotic stresses, In previous reports mostly researchers used seedling to apply stresses for the analysis of the expression levels, but I was considering using the passion fruit peel or pulp as material after biotic and abiotic stresses for qPCR expressions,
1- which material will be recommended, Peel or Pulp or both?
2- For biotic stress, I considered applying different pathogenic fungi on peel and will collect the peel samples after rotten occurs (5-7 dpi), and control samples will be without fungus infection.
So that will be fine?
2- And for abiotic stresses which parameters are more suitable - low or high temperature, or should be both? according to reports, the storage temperature of passion fruit is 3-5 C, so for abiotic stress should be used temperatures? For example; 0, 2, 4, 6, 8 C, and 4 C will be considered as control, so is this all right?
Your suggestions will be welcome
Regard
Rizwan