Hi Hafiz.

Do not be overconfident with the excel spreadsheet. There you will have some compound names associated with a match percentage (be careful with this R match). This means it is possible to end up with misidentified compounds. Good thing here is that you will have some total areas for your compounds that could be quantified. That said, if you have standards for your wax, you can just not only effectively identified it by comparing their retention times, but to generate a calibration curve to quantify it.

Anyway, the best thing to do to analyze the data, for determination purposes, is to work directly with your GCMS software and confirm the fragmentation patterns of your compounds.

Good luck

What you do with it depends what you want to know.

If you are trying to characterise the wax then I completely agree with Amulfo that you should not take the identification of compounds within the software as definite. Always check the percentage match. Anything under about 85% you should double check. I usually check everything as there are a few common compounds the library always mis-matches. You can see this quite easily from your results where there are two compounds both identified as C16H32O2, but different compounds - which they will be as they are eluting at different retention times. The atoms will be arranged differently for each one and they will be different compounds and the only way to check is to look at the fragmentation patterns in the mass spectrum. You also have several peaks identified as hexadecane - this is unlikely, expecially as some are at very different retention times. This is common with alkanes as the molecular ion is very small or sometimes missing and the software easily misidentifies them. They are probably all alkanes but will be different ones.

For the mass of peaks which have not been identified you will need to work on the mass spectra to see if they match anything in the literature, or at least belong to the same family of compounds as things in the literature. You can narrow that down by looking at compounds which commonly occur in cuticle waxes - alkanes, alcohols, fatty acids, wax and other esters etc. Other than that you can look at the fragmentation patterns and see if you can identify compounds using those. This is fairly complex, but can be done with the help of a good book on the subject.

Someone who has been doing GC-MS analysis for a while should be able to help you with this. It is a vaste subject and it sounds as though you need some guidance.

The siloxanes may not be part of the natural wax (in fact are unlikely to be). They are more likely to be from your GC-MS column or the lid of your vial.

You won't be able to do quantification except in percentage terms as you have not added an internal standard.

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601259/pdf/11101_2012_Article_9241.pdf

https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.5b00013

https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1000777

Thanks