I am working on Okra, during my research work i have experienced that after inoculation of hypocotyl and cotyledonary explants into full MS medium supplemented with different PGRs, callusing starts ,with in1-1.5 month the callus became whitish compact but with the passage of time on the same medium, callus stars the light greenish color, so my question is that :
1. Is it possible that by the use of modified MS medium, may can convert the whitish, compact non-embryogenic callus to greenish , friable embryogenic callus which will more efficient for shoot induction?
(Pictures attached for reference)
Note: If you have experience in it or have read any article then kindly share with me (focusing on role of nitrogen on callus / shoots induction in MS medium )
Thanks in advance
Dear Hafiz
The combined effect of 2.0 mg/l 2,4-D with 1.0 mg/l NAA gave the best response for embryogenic callus induction on the media with MS salts, B5 vitamins, 30 g/l sucrose and 0.8 % agar. At this concentration, only creamy white friable embryogenic callus was obtained.
M. GANESAN, R. CHANDRASEKAR, B.D. RANJITHA KUMARI and N. JAYABALAN Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture. BIOLOGIA PLANTARUM 51 (3): 414-420, 2007
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