Dear Researchers:

I want to do an experiment about the cuticle wax chemical composition and concentration of fruit surface at the different developmental stage so I would like to ask some questions about GC MS:

1- What criteria should keep in mind while selecting an Internal standard?

2-How many internal standards should use? any limitations for Min/Max?

3- What about the IS concentration? concentration should use only one concentration or use at different concentrations?

3- Please recommend some IS about waxy compounds (VLCFAC) such as C7 - C40 Saturated Alkanes Standard?

Regard:

Rizwan