Does anyone have a good protocol for genotyping APC flox mice from Jax (https://www.jax.org/strain/009045) C57BL/6-Apctm1Tyj/J? I am also looking for a std. PCR assay since the genotyping protocol from JAX may be a qPCR-based assay and did not work in our hands. Thanks,
Jianning Tao
we will try your magic tips and let you know if it works in our hand. Thanks,
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Subhash C. Juneja
To be honest, the jackson protocol looks very abnormal protocol to me
One time I was feeling very difficulty in one of PCR, I tried touch town, Anealing 65C to 58C and decreases slowly, I am am enclosing that protocol, you may try on the similar to modify from 68 to 50 for your experiment, please see page 2 of enclosed protocol. Good luck
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