Hello all,
I'm interested in testing the cardiotoxicity of different drugs of interest using a Ca2+ influx assay, specifically the Molecular Devices EarlyTox cardiotoxicity kit that uses fura-2 based dye and read on a SpectraMax microplate reader. So far, we've been using iPSC-derived cardiomyocytes (iCells), but we are interested in isolating cardiomyocytes from adult mice instead. Does anyone know of any good protocols and whether isolated mouse myocytes have been used before in a Ca2+ influx assay?
Hi
we published the protocol to isolate adult mice cardiomyocytes, but never used the kit you mentioned. You can find our protocol on JoVE:
Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes. J Vis Exp. 2013 Sep 24;(79). doi: 10.3791/50289. PMID: 24084584
Hope it helps
Hi, we have worked with mouse cardiomyocytes. Most of the time we used them in electrophysiology experiments and intracelular calcium measurements and cells work well. If you want to try I'm attaching a short version of the protocol.
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