I would like to feed STZ-injected mice (type 2 diabetes) on cholesterol diet for 28 weeks. Is this period enough to induce atherosclerosis? since wild type mice are resistant to atherosclerosis
I think it is more than enough time I have seen artheroscllerosis develop in diabetic mice in 24 weeks.this artile may help
Effect of Streptozotocin-Induced Hyperglycemia on Lipid Profiles, Formation of Advanced Glycation Endproducts in Lesions, and Extent of Atherosclerosis in LDL Receptor-Deficient Mice
Peter Reaven, Shiva Merat, Florencia Casanada, Mary Sutphin, Wulf Palinski
The wild-type C57BL/6 mice supplemented with saturated fat, cholesterol, and cholate develop only a moderate lesion. An alternative to the ApoE and the LDLr KO models is the new developed AAV8-PCSK9.
Wild type will not develop atherosclerotic, no matter the type of intervention you will use. You must use modified strains of mice, as the ones pointed out by the previous commentaries