I need to know what precautions need to be taken for using CCl4 in the mouse model of inducing liver fibrosis. I plan to inject the CCl4 intraperitonealy. The biosafety comittee has questions as to the toxicity of the CCl4 to laboratory and animal care staff.
we can induce liver fibrosis by diethyl nitrosamine IP weekly injection for 6 weeks. you should wear gloves when using DEN or ccl4
Ibrahim El Sayed - I think it is usually important to study the effect on two models rather than one, so that you show that the effects are not related to CCl4 effect, but the pathological changes in the liver.
Complications could be development of ascites, gastritis, bleeding, cirrhosis or cholestasis occur or animal death.
The model in mice is not identical to human liver fibrosis/cirrhosis. See this publication for example. CCl4 is toxic and you have to follow toxicological lab regulations.
BMC Gastroenterol. 2010 Jul 9;10:79. doi: 10.1186/1471-230X-10-79.
Mouse model of carbon tetrachloride induced liver fibrosis: Histopathological changes and expression of CD133 and epidermal growth factor.
Fujii T1, Fuchs BC, Yamada S, Lauwers GY, Kulu Y, Goodwin JM, Lanuti M, Tanabe KK.
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