Can anyone suggest for me the best LPS can be purchased from Sigma to induce asthmatic mouse model intratracheally?

07 July 2019 1 9K Report

I found different LPS from E. Coli with different serotypes. Is there any difference between O111:B4 serotype and O55:B5?

I would appreciate if someone could provide me with a good protocol of inducing asthma in mice using LPS.

Thanks

Alireza Mordadi

Unfortunately I haven't had any experience in this but absolutely different serotypes active immune system,s pathways in different way so seek for pathways and check with your goals.

Regards.

Badges
Science topic

More Lamis M.F El-Baz's questions See All

Double-Staining of MPO and histones with SYTOX Green and DAPI?

Double-Staining of MPO and histones with SYTOX Green and DAPI

22 April 2024 5,516 0 View

Whats a good Software for complex designs of microfluidics chips ?

Hello everyone, I'm looking for a software thats adapted for drawing complex designs of microchannels. Typically i have 2 inlets, 3 outlets and 100 microchannels in between. For the time being, i...

17 October 2021 5,699 4 View

Flow cytometry antibodies (conjugated with PE or FITC) work for Immunofluorescence µscopy ?

Hello, im trying to visualize surface antigens of lymphocyte cell line cells with primary antibody immunoFluorescence. I cant have a proper protocol about this issue. I followed flow cytometry...

28 October 2020 5,058 3 View

What is the best treatment for refractory ascites caused by liver cell failure?

any guidelines for refractory ascites?

08 February 2016 8,193 5 View

Has any research on TOC (theory of constraints) been carried out in developing countries?

Has any researcher from africa or asia studied Theory of Constraints?

16 February 2015 2,932 5 View

Which Co2 incubator is the best for stem cell cultures: water-jacket or air-jacket?

stem cells- tissue culture- cell culture.

09 October 2014 3,346 1 View

Double-Staining of MPO and histones with SYTOX Green and DAPI?

Double-Staining of MPO and histones with SYTOX Green and DAPI

01 January 1970 9,185 0 View

Young's Modulus of unique cells

Many tools exist to determine the elasticiy or Young's Modulus of cells. However, sometimes when we compare data obtained by the same approach (By indentation using AFM for instance), we see data...

01 January 1970 5,604 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Why can't academics earn the money they deserve?

Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...

01 August 2024 6,469 6 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

Where to download DEM from 2018?

I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...

31 July 2024 9,757 3 View

SAS Generalized Linear Model for trial/event anaysis and not survival (time to event) analysis?

I am looking for a published article using SAS or SPSS Generalized linear model for trial/event data and not survival analysis. Both software packages off the option for the number of success out...

30 July 2024 3,835 2 View

What are the perfect mathematical model for a solar-powered ship implementation?

A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids

28 July 2024 4,985 1 View