The differentiation protocol of mouse CD34+ bone marrow cells into dendritic cells is well known. Is anybody familiar with protocols describing the differentiation of mouse CD34+ BM cells into other immune cell types (e.g. T and B cells, NK cells, etc.) ?
http://www.bloodjournal.org/content/101/11/4313?sso-checked=true
This is an older paper but describes their method for differentiating CD34+ HSCs into various immune cells. This is with human though.
These protocols used previously in similar papers:
For B-cells
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783328/
https://www.ncbi.nlm.nih.gov/pubmed/8989909
For T-cells
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191035/
For NK cells
Article Generation of natural killer cells from hematopoietic stem c...
Take care that these articles are using human CD34+ cells,
Hope this helps
Hello
Kindly look at these link
Article In vitro and in vivo differentiation into B cells, T cells, ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303970/
Article Generation of natural killer cells from hematopoietic stem c...
Article Comparative Study of Hematopoietic Differentiation between H...
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