Can anyone provide a protocol or source for how to easily obtain plasma membrane sheets?

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Why is ΔHmix for elemental phase small-negative?

For elemental phase (= terminal solid solution based on one metal element) consisting of one major element, Yeh et al. [1], Murty et al. [2] and Gao et al. [3] proposed a small-negative enthalpy...

21 January 2023 522 4 View

1H NMR: peak indentifcation 1.79 ppm in bacteria infected urine?

Hi, I did some 1H NMR measurements to analyse bacteria-infected urine (yellow graph). I have a clear peak at 1.93 ppm (according to literature: acetic acid). However, I cannot identify the peak...

31 March 2022 2,179 1 View

How long are the average drying times of nectar in honey bee comb cells?

Is there any publication that has investigated how long it takes for nectar to dry within a cell inside the hive, before it is capped? I am aware that it depends on multiple factors such as the...

31 January 2022 439 2 View

In Abaqus, is it possible to remove elements which are too complex for meshing from a sequence obtained by getByBoundingBox() ?

Hello everyone, I'm trying to mesh a structure with complex partitions using mesh controls. When doing it on the user interface I'm first selecting all cells. In the following, I have to accept...

03 February 2021 8,600 1 View

How to compare expected and counted values statistically with different p values for each genotype?

Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...

03 February 2021 6,753 2 View

Can a mixed background in mice reduce cre recombinase activity in comparison to a pure Bl6 background?

Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...

24 September 2020 2,060 3 View

LPS- B cell activation only works once in a while??

I was wondering if anybody could give some tips for B cell culture (primary splenic B cells, MACS purified, 5x10^5/ml + 8ug/ml LPS, + 20ng/ml IL-4, in RPMI, 1-2 days). It seems that I can use...

13 July 2020 6,596 0 View

Bibliometric analysis – How can I complement Web of Science data with additional data for co-citation analysis?

Dear all, I have the following problem: I want to run a co-citation analysis of a certain set of publications. However, some of them are not part of the Web of Science database. Is there a...

13 December 2019 9,839 7 View

How to increase efficiency of lentiviral transduction in primary neural cells?

I am trying to transduce primary murine cerebellar granule cells using a lentiviral system. I tried transduction with polybrene, but it seems to be quite toxic for my cells. However, transduction...

10 November 2019 8,691 6 View

Buffers needed for Annexin V staining?

Hello you all, we want to perform an Annexin V and PI staining on our B cell culture, does anybody know if using the commercial buffer (biolegend in our case) is absolutely necessary? Or can we...

14 May 2019 2,733 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View