Dear John,

I guess it should be possible to show the orthogonal views in FIJI and then add the scale bars in the xz and yz panel.

Best,

Andreas

Dear @John Brooks,

My response is perhaps belated but I'm agree with @Andreas Müller for the use of the orthogonal views generated by ImageJ or Figi (linked to this message, a picture with an example of vertical and orthogonal views).

If you want more informations about this tool, you can check this website : https://petebankhead.gitbooks.io/imagej-intro/content/chapters/dimensions/dimensions.html

Hi John Brooks,

I assume you used 3D viewer plugin for constructing the 3D. However, it is indeed challenging to add scale bar in such condition. What is the best (in my opinion) is that you use the 3D projection instead. Go to Image > Stacks > 3D Project. Choose axis of rotation that you prefer (x or y or z). And later, before adding the scale bar, you can transform the position of the image according to your preference. Go to Image > Transform > Rotate. And finally, you can add the scale bar.

Hope that helps.

Royan