How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Hi. How can I carry out mediation analysis on AMOS without all items loading onto each construct? Should I calculate the mean and proceed?

Hi, I am trying to run mediation analysis on AMOS. In the tutorials there are no items loading onto a factor which is not the case for the development of a measurement and structural model. How...

23 October 2020 4,240 8 View

How to detect Methanogens using Flow Cytometry from wastewater?

Hi everyone! I'm trying to identify methanogens from wastewater using flow cytometry . Any idea on how to go about it? which stains or flurochromes we have to use? how do we go about isolation of...

24 June 2020 3,906 4 View

How can I use AMOS to conduct Exploratory Structural Equation modelling?

Almost all studies have used Mplus for Exploratory structural equation modelling ESEM. I wonder if it is possible to use AMOS for this purpose. Can I get a reference which discusses the specifics...

12 May 2020 5,269 8 View

What is the difference between spark plasma sintering and electric resistance sintering?

It is well known that plasma sintering is based on the electrical spark discharge phenomenon for preparing high-density composites at lower temperatures and higher sintering speed. How it differs...

03 March 2020 4,458 4 View

Plasma Nitriding with hollow cathode discharge?

We have a cathodic arc PVD system which uses hollow cathode arc (plasma) with Ar as a prior cleaning step. Now our idea is to use this set up for plasma nitriding of SS 3xx series. The plan is to...

06 February 2020 5,596 1 View

What are the best environmentally benign/green solvents to dissolve PMMA?

I would like to use environmentally benign solvents for dissolution of PMMA to make thin films and coatings.

10 November 2019 8,847 4 View

Questions regarding adapted questionnaire?

I HAVE FEW QUESTIONS REGARDING ADAPTED QUESTIONNAIRE. IF ANYONE KNOWS ANY OF THEM, PLEASE ANSWER is it necessary to adapt from some journal's article? or we can adapt from thesis or review...

09 November 2019 6,303 6 View

Is there a possibility of Microcarburizing environment in graphite heating furnace?

I am planning a sintering experiment for an Fe-Si system. I have a high temperature furnace with graphite heating elements. I am concerned if there's a risk of carbon pick up due to prolonged...

28 August 2019 2,927 1 View

Photoelectrochemical study of CdS, CdTe and CdSTe thin film, which electrolyte i must use and which must be the counter electrode?

I have prepared CdS, CdTe, CdSTe tgub films and now doing its PEC study. I tried it with polysulphide electrolyte but the Isc and Voc obtained was very less than expected. again I tried with...

13 August 2019 6,727 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View