How can I differentiate between THP1 and Machrophages cell in the flask?

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Please guide me how to prepare samples for zeiss confocal microscope?

i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...

30 July 2024 2,349 1 View

Human macrophages cell surface markers?

I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...

29 July 2024 236 2 View

Steingrimur Stefansson

Hi Ebtisam, if I remember correctly, THP-1 cells can be washed away from the plate whereas macrophages stick tightly.

Mosab Nouraldein Mohammed Hamad

https://www.google.com/search?

https://www.google.com/search?biw=1366&bih=651&tbm=isch&sa=1&ei=YejHWun8HcrUwQKA6bWIBA&q=macrophage+under++microscope&oq=macrophage+under++microscope&gs_l=psy-ab.12..0.55146.61399.0.64510.14.14.0.0.0.0.637.2049.4-3j1.4.0....0...1c.1.64.psy-ab..10.4.2047...0i67k1j0i7i30k1j0i13k1.0.6E4k5qv793Y

q=T+helper1+under++microscope&source=lnms&tbm=isch&sa=X&ved=0ahUKEwjL45nJzabaAhUDaFAKHRBZBtcQ_AUICigB&biw=1366&bih=651

Hi Mosab, can you distill your answer into a paragraph of your own making?

I get annoyed when people answer a question with an avalanche of hyperlinks and expect the person posing the question to sift through everything.

This is lazy. If you don't have a specific answer to a specific question then please don't post general and non helpful links.