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Questions related to Extraction
I treated SK-N-SH cells with 1mM H2O2 and 1mM H2O2+antioxidants (3 different mixtures) and measured the mitochondrial membrane potential (ΔΨm) with 500nM TMRE. As far as I know, H2O2 and ROS...
19 October 2021 2,703 14 View
Hello, everyone..I got the same issue as I begun a MD it finished and then got to "Not responding mode", but I understand and still waiting for it to finish. I got VMD/Namd2 for MD, yet I do...
16 October 2021 6,824 4 View
Hi guys, I am working on a project using HS-SPME to extract volatile compounds. I am wondering what would be the best procedure to prepare n-alkane standards and grob mix? like what solvent should...
15 October 2021 1,527 0 View
I am looking for a company that sequence phages. I need DNA extraction also. Any suggestion is welcome.
04 October 2021 2,151 3 View
the protocol i am using for RNA extraction states i should spin at 12000 rpm for 1 min at some steps, however, the centrifugation device i am using reaches that speed after 1 min of hitting the...
03 October 2021 6,257 4 View
is LC/MS MS appropriate for antigen detection?
17 September 2021 4,030 2 View
Hi, I am working on wind turbine aerodynamic and I want to know if the stability of an aerodynamic body like an aircraft can be applied in the wind turbine field. If you have any ideas or...
14 September 2021 5,553 4 View
Hello I wish you good health I have a question: In different articles, different volumetric ratios have been used for DPPH testing. For example, one paper used 0.5 cc of sample and 2.5 cc of DPPH...
19 August 2021 6,795 14 View
Can I use GC-MS instead of LC-MS for confirmation of synthesized benzyl mercaptan adducts (catechin-BM, Epicatechin-BM, Gallocatechin-BM, and Epigallocatechin-BM)? I can't seem to find methods...
14 August 2021 8,177 6 View
Following uptake experiments, we want to lyse macrophages without extracting the plasma membrane. Liquid nitrogen treatment with hypotonic buffer would appear to be a suitable method - does anyone...
10 August 2021 6,455 0 View
I am working on the adsorption of Lead (Pb) and Copper (Cu) on clay minerals under influence of dissolved organic matter (Extracted from the soil). My objective is to exactly estimate the above...
08 August 2021 4,657 5 View
I am carrying out Western blot to determine effects of extracts on expression of LPS induced iNOS in murine macrophage cells, I am using the iBind flex system from Thermofisher for multiplexing...
04 August 2021 7,085 0 View
Hi, I am trying to model the Schottky diode SMS 7630-079 LF for rectifier application in ADS for a frequency of 2.4 GHz. The datasheet provides absolutely no information apart from the basic...
28 July 2021 3,277 1 View
Good day everyone, I need an assistant to learn how to search for relevant materials discuss some of the physical, chemical and biological of soil in urban forests in the UK context. I look...
24 July 2021 3,336 5 View
Hi everyone, I have Covid infected tissues and have to isolate RNA from them. But I dont have an access to BSL3 or 4 laboratory in my institute. My colleagues are prepared and fixed tissues in...
01 July 2021 6,809 5 View
I put the S parameter in CST Design Studio and set it to the active S parameter, but how do I add the bias voltage to it.And should I add a discrete port to the CST MWS to connect Touchstone to it?
01 July 2021 6,229 3 View
Can the hypersensitive response occur as part of PAMP-Triggered Immunity (PTI) as well as Effector-Triggered Immunity (ETI)?
24 June 2021 4,395 3 View
I am working on a project related to aflatoxins. To estimate the quality and quantity of aflatoxins by analytical techniques. I want to destroy the fungal spores which could contaminate the...
22 June 2021 4,891 15 View
The mass of analyte found in the sample is corrected for DE. So if the DE value is low but stable, will it be ok to use the value of 40%. I increased the number of extractions by 2 and 3 times and...
20 June 2021 6,194 0 View
I need a dye that wouldn't interact with the ascorbic acid to be encapsulated as a marker for measuring the encapsulation efficiency using the UV spectrometer. I have tired to use some dyes but it...
15 June 2021 2,567 5 View
Could you please explain the differentiation of aninal to meat with percentage wise distribution. Carcass yield, bone %(live wt, carcass wt), meat%
12 June 2021 4,285 7 View
the first two lines are cell lysate samples, the 3rd and 4th line are my exosome lysate samples isolated from the cell culture medium. I isolate exosome with Total Exosome Isolation kit from the...
10 June 2021 8,516 3 View
I am thinking of using kits for extraction of DNA and other genetic materials from water
07 June 2021 1,830 5 View
I am currently growing Moringa trees to supply leaves, roots to companies which can use it for medical uses. Any suggestions on what tests need to be done before I supply them to the companies and...
01 June 2021 5,613 11 View