Extraction

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Questions related to Extraction

Can I use QIAamp DNA Stool Mini Kit for DNA extraction from a bacterial colony? if anyone tried this and what are the necessary protocol adjustments?

#DNA_extraction #QIAamp #Qiagen #Colony_DNA

05 May 2023 4,532 2 View

Sanger sequencing for unknown microbes?

We have 150+ glycerol stocks of unknown microbes (from soil) that we need sequenced and we have tried sending them off for direct colony sequencing using universal 16S primers and it failed (even...

29 April 2023 5,541 3 View

Advantages of oxalic acid over sulphuric acid in nanacellulose extraction ?

In the acid hydrolysis step of nano cellulose extraction , recent literatures are citing oxalic acid treatment in comparison with sulphuric acid

27 April 2023 6,007 2 View

Does anyone want to collaborate?

does anyone want to collaborate on the optimization of extractino technique for bioactive compounds from a plant ?

26 April 2023 2,294 2 View

Partition coefficient for sterols ?

Hi! I'm doing a research about sterols extraction. I've been struggling to find the partition coefficient factor for sterols using diethyl ether, if you have any source that can help me please let...

25 April 2023 9,527 0 View

Is there any Online database of phenolic compounds? (Except phenol-explorer) ?

I am searching for an online database of phenolic compounds extracted from plants which contains their UV spectra (or at least their λmax). In the database phenol-explorer, there is almost...

25 April 2023 3,769 3 View

Is it possible IC50 value of standard ascorbic acid is 0.2030 microgram per ml?

I prepare different concentration of ascorbic acid such as 80 migram per ml, 40,20,10,5,2.5,1.25,0.625 and absorbance measured by uv spectrophotometer at 517nm....

20 April 2023 2,558 4 View

Which mEOS version to use for PALM?

Hi, I would like to use mEOS to tag proteins to evaluate their subcellular localization. I am new to PALM, and am a bit confused with all the different versions of mEOS available... mEOS2? mEOS4?...

20 April 2023 7,440 1 View

How do random hexamers work for qPCR?

Hello, We have always used random hexamers in my lab when creating single-strand cDNA for qPCR applications. My understanding is that these primers bind to the RNA and reverse transcriptase...

18 April 2023 7,220 3 View

What is the current state of art for audio emotion detection?. Kindly provide info on industry ready datasets and paper. Please try to accompany code?

what is the current state of art for audio/audio recording/speech emotion detection?. Kindly provide info on industry ready datasets and paper. Please try to accompany code.?

18 April 2023 8,476 0 View

Samoene can give me the liste of the main phenolic and flavonoids used as Standards in HPLC-UV PLEASE ?

I want pruchase the standards for hplc from Sigma aldrich for study the chemical Composition of some plant, and i want the widlely compounds used

17 April 2023 4,740 4 View

Is it possible to detect PAHs directly from microplastic particles found in fish guts and gills?

I have already processed my fish gut samples for microplastics analysis from extraction to filtration and drying. The extracted microplastic particles were stored in dried glass fiber membranes....

17 April 2023 8,452 2 View

Where can I buy polyethylene microplastic with the particle size ranging from 1 um to 5 um?

Preferably, in the Philippines.

12 April 2023 1,734 2 View

How to obtain serum or plasma from haemolysed blood?

Hi have whole blood samples that have been freezed at -80 without any previous separation. Now all the samples are haemolysed. Does it exist any protocol to obtain plasma or serum from these...

06 April 2023 3,574 0 View

How do you find the unreported sample size from given the means and standard deviations of two study groups?

04 April 2023 6,613 6 View

Is it technically correct to simulate a single chain of protein of a multimeric protein?

I am interested in the molecular dynamics simulation of a protein. The protein is homotetrameric and each chain contains more than 250 amino acids. I want to measure the changes in the protein at...

01 April 2023 2,513 3 View

How to generate daily weather for CMIP6 AR6 data?

Greetings to all the researchers around the world! I have a few queries regarding climate change modeling. Any info regarding this would be highly appreciated. A few weather generators are...

30 March 2023 872 2 View

Can I dissolve plant extract into methanol or DMSO after extracting using n- hexane as a solvent?

I extracted my phytochemicals using three solvents methanol, n-hexane and ethyl acetate. After filtering I dried them in Room temperature Now I want to know if I can make stock of these extracts...

28 March 2023 2,815 1 View

Help with study methodology for clinical studies?

Hello everyone, I have some questions. Writing a paper and trying to determine what is study methodology. 1. Study was conducted over 1 year period, they included hospitalized patients poisoned...

24 March 2023 977 0 View

Can you be our speaker?

Negros Oriental State University, in the Philippines, will conduct an online seminar about "Embedding Gender Issues in Research" on April 14, 2023 from 1:00 PM to 5:00 PM Philippine Time. We need...

20 March 2023 9,421 0 View

Is it okay to use ethanol to dilute plant extract for antibacterial test? Do ethanol has some toxicity that can give the false positive result?

I want to dissolved my ethanol extract with ethanol to make different concentration for antibacterial test. Or is there another solvent that can be use for dissolving plant extract? And why is it...

15 March 2023 2,640 5 View

Is there any standard protocol for plant allelopathic potential assessment?

Researchers usually simulate allelochemicals release pathways ( volatilization, rain and fog leaching, root exudate, residue decomposition ) through different allelochemical extraction methods....

14 March 2023 6,604 0 View

Which solvents could contribute to a realistic measurement of the hydrodynamic diameter via dynamic light scattering (DLS)?

Good evening, I am currently characterizing the surface chemistry of functionalized zirconia nanoparticles synthesized from different approaches. In the final step of my investigation I measured...

08 March 2023 8,781 3 View

Do you think it is a good idea to publish a book chapter through IntechOpen?

I was invited to publish a book chapter in IntechOpen. I read the book project and it seemed to be fascinating. But, I was unfamiliar with the publication, and I am skeptical of its authenticity....

08 March 2023 722 1 View

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