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Questions related to Extraction
I need to measure the biological activity of the cytokine Interleukin 2 (IL-2) by its effect on CTLL-2 cells so that I can determine its EC50. I was informed that the specific activity of IL-2, in...
15 January 2024 2,521 4 View
Where are sustained mathematics errors in RG Research Interest Score computations and sustained discrepancies in RG site and RG Apps, as well as biasing in measuring of citations.
14 January 2024 5,514 0 View
I am trying cloning of my gene of interest in pet28a vector and trying to transform it in DH5a. But after transformation I got this type of plate. Can anyone tell me what is the problem here?
13 January 2024 6,706 4 View
For my perovskite material I have got this XRD pattern (see the attachment). The baseline subtraction has already been done but still the prominent peaks are not arising from the baseline. I want...
12 January 2024 6,770 6 View
We are currently cultivating 4G2 cell lines in DMEM supplemented with 10% FBS. We’ve noticed some mobile particles in the culture media, which we initially suspected to be bacteria. Despite the...
11 January 2024 3,632 0 View
How to calculat flux recovery for nanofiltration membrame?
11 January 2024 4,267 0 View
How do I do molecular docking of ice interface and polysaccharide? use what software?
11 January 2024 4,318 0 View
Kindly explain, how to calculate the elemental composition of weight and atomic percentage from EDX. Does anyone know the formula to calculate the at% and Wt%?
10 January 2024 1,562 3 View
I do ELISA many times, and my result is not the same. I realize that when I harvested the supernatant, the volume each well was different. For example I stimulate cells with 200 ul media, when I...
09 January 2024 5,696 4 View
Why in an ecosystem is energy said to flow while nutrients are said to cycle and how are nutrients recycled in an ecosystem?
09 January 2024 8,013 0 View
Preferably one that can be done by a student in a lab setting with medium facilities. I need it for my project.
07 January 2024 7,994 0 View
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04 January 2024 7,336 2 View
04 January 2024 7,370 1 View
We're trying to get cross-sectional SEM images of alkali metal electrodes (Li, Na). we cut by our lab-knife or lab-scissor as neatly as possible, but results were unsatisfied. Is there any...
04 January 2024 7,129 2 View
03 January 2024 4,678 2 View
I have to occasionally etch tantalum to evaluate grain structure, but I often times find the etchant doesn't fully reveal the grain boundaries. Perhaps using a color etchant as well is worthwhile....
03 January 2024 8,641 0 View
Which bacteria convert nitrogen into usable form in industry and bacteria converts’ atmospheric nitrogen into a nitrogenous compound?
01 January 2024 8,730 3 View
a l instar des changements climatiques ,quelles sont les principales maladies émergentes er re émergentes au Nord africain ?
29 December 2023 4,297 1 View
I need to explain the mechanism of biotransformation of guaiacol in tetraguaiacol biocatalised by a plant peroxidase and I might know if the pathways of this mechanism differs or not differs from...
28 December 2023 2,798 0 View
What are the correct values of molar concentration of O2 (Co) for the Koutecky-Levich plot in 0.1 M H2SO4?
18 December 2023 1,301 0 View
I have XRD data of my synthesized hydroxyapatite (HA) and I want to compare it with standard HA peaks
14 December 2023 816 1 View
I am a master's student in Korea. I have a question while conducting a cell culture experiment on PDMS. What should I do to allow HaCaT cells to grow on PDMS without O2 plasma treatment during...
13 December 2023 6,637 0 View
Why is it that my calculations using the global land cover dataset version 30m2020 some countries have a lot more impervious surface area compared to 2010 and a lot of them are anomalies, and also...
13 December 2023 4,786 3 View
Could you please advise on the optimal amount of exopolysaccharide to extract from a 500 ml bacterial culture?
13 December 2023 9,465 0 View