Extraction

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Questions related to Extraction

Materials studio C11小于C12为什么?

尊敬的各位我在使用MS软件中的castep计算一个铁基二元合金的弹性常数时计算结果中的C11要小于C12请问这是什么情况？

06 December 2024 1,679 3 View

What are the possible causes for high cytokine background in untreated PBMC culture?

I have isolated fresh PBMC from whole blood using Histopaque 1077 and seeded onto a 96 well plate and incubated for 16-20 hrs in CO2 incubator. Few cytokines & chemokines secreted from the...

19 November 2024 5,253 3 View

'Pre-coating' ELISA dilution plates with PBS (Gibco vs. Sigma differences)?

I have been working with a 96-well ELISA, in which I perform serial dilution of 8 standards in wells of a non-binding plate from Grenier bio-one (#655901) before transferring these to the assay...

18 November 2024 9,708 0 View

Why does the background of an oxide film oxidized under atmospheric conditions increase?

304 stainless steel was oxidized using an induction coil under the following conditions: atmospheric atmosphere at 900°C, steam atmosphere at 900°C, and steam atmosphere at 1000°C. After the...

18 November 2024 3,868 6 View

How to detect and analyze compounds with little or no UV absorption in LC purification?

How can compounds with minimal or no UV absorption be effectively detected and monitored during liquid chromatography (normal or reversed-phase) purification? I am particularly interested in...

15 November 2024 9,979 6 View

Is HUVEC cell sensitive to glucose level?

Hello everyone, I have some doubts about the HUVECs. Is this cell sensitive to the glucose level of the culture medium? Does high glucose level induce harmful effects on HUVECs?

14 November 2024 5,003 2 View

What is the best way to cryopreserve tumors for later cryosectioning and immunofluorescence?

The cryopreservation protocol I am familiar with is as following: 1. PFA 4% overnight at 4C 2. WashX3 cold PBS 3. Sucrose 15% at 4C until submerged 4. Sucrose 30% at 4C overnight 5. OCT...

06 November 2024 1,754 2 View

Interested in old 2011 ideas on the road towards agricultural sustainability that was supposed to BEGIN from RIO +20 2012? Have you read this article?

Muñoz, Lucio, 2011. The Present versus the Future in development thinking: Towards Agricultural Sustainability, Journal of Sustainability, Issue 3, Number 3(Winter), Rio Rancho, New Mexico...

05 November 2024 7,372 2 View

Can you please recommend a good protocol for Silver Nitrate staining for DNA samples run on SDS page gels?

Can you please recommend a good protocol for Silver Nitrate staining for DNA samples run on SDS page gels? We have also tried this with protein samples however we couldn't see any bands. So, the...

03 November 2024 8,912 0 View

Difficulty validating UPLC-MS/MS method for masters thesis?

Hello smart people. I can to you in my time of need. I am at wits end trying to validate my methodology to move forward with my thesis lab work dealing with environmental concentrations of...

31 October 2024 4,662 0 View

Ames testing in Canada?

I am looking to do some Ames testing throughout my research however I have struggled to find Ames tester strains available in Canada. I was able to find some through Cedarlane however the prices...

29 October 2024 6,044 0 View

Help regarding XCMS?

I have converted .raw file generated from Orbitrap HRLCMS (negetive ion mode) to mzXmL and submitted as a single job to XCMS. Not getting proper parameters for LCMS, can anyone help me which...

29 October 2024 9,931 1 View

Seeking guidance on Jurassic-Cretaceous fossil plants used in stratigraphy?

Hello, I am a PhD student in sedimentology and biostratigraphy, and I have a fossil leaf specimen from a Jurassic-Cretaceous deposit. I would like to receive recommendations on fossil plants used...

25 October 2024 914 7 View

Are there any studies showing the absorbance/transmittance and reflectance curves of the iron oxides used in sunscreens?

I want to analyze the isolated behaviour of the three iron oxides (black, yellow and red) usually added to sunscreens versus traditional organic sunscreens without the addition of the pigment in...

24 October 2024 7,413 2 View

Does GlycoBlue affect mass spectrometry with RNA samples?

Hello everyone! Currently, I am preparing RNA samples from a minimal material. I would like to use GlycoBlue to increase the yield and make it easier to work with the pellet. Do you know if the...

24 October 2024 1,353 5 View

What is the best conditions for formation Sn porphyry deposits with highest grade of ore?

I want to know more about Sn porphyry deposits.

22 October 2024 2,835 1 View

Do you know best composition of best bentonites?

I want to know more about bentonites.

22 October 2024 8,063 1 View

What is the decomposition temperature of metal organic framework?

I prepared a metal-organic framework (MOF) modified TiO2 material at 500 centigrade the EDS and XPs results showed the existence of main elements like carbon, nitrogen etc that come from MOF. As I...

17 October 2024 537 3 View

Request for Collaboration and Peer Review on Soil Stabilization Using Metallic Tailing Powder?

Hello colleagues, I am currently working on the development of soil stabilization techniques using metallic tailing powder as a key component. I have completed a research article on this topic and...

09 October 2024 2,989 2 View

How can software be optimized to make better use of hardware resources?

Optimizing software to make better use of hardware resources is a complex task that involves multiple layers of the software stack, from application level to system level.

01 October 2024 5,051 0 View

IC50 of temozolamide in human glioblastoma multiforme?

IC50 in vivo invitro

25 September 2024 7,407 2 View

What Could Be Causing Contamination I see in my Human Mammary Epithelial Cell Plates?

Hi everyone, I'm encountering contamination in my Human Mammary epithelial cell cultures, but I’m unsure of its nature. After passaging, I plated cells from the same batch into 8 plates, but the...

20 September 2024 8,699 0 View

What is the most important different of metamorphic garnets with igneous garnets?

I want to know more about garnets.

20 September 2024 5,174 3 View

Why does guaiacol oxidation occur on plate assay but not in laccase enzyme assay for the same microbial culture?

I am working on laccase production from fungi and bacteria. During plate assays, I observe a reddish-brown color forming around the colonies, indicating that guaiacol (used as a substrate) is...

17 September 2024 7,046 1 View

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