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Questions related to Extraction
I'm growing Pichia pastoris in BMGY medium currently and I'm trying to follow, how it grows, so I measure OD600. But as I expected the yeast to grow (and hence increase OD600), I diluted it more...
29 July 2023 9,382 3 View
Can a biofilm on a boat hull for example, corrode metal on one surface and be an attachment site for marine invertebrates on a different surface at the same time? Or does one event change the...
29 July 2023 3,146 5 View
I have tried multiple protocols with varying ratios of DCM and SDS but was not successful in preparing PLA emulsified Agar media. Problems faced , 1. Plates are too transparent to see any...
28 July 2023 850 2 View
Additionally, what factors should be considered when selecting an appropriate image processing algorithm for lineament extraction?
28 July 2023 4,936 3 View
For LOD and LOQ I need a *blank sample* and *sample of known concentration*. For the *blank sample*, should I just fill the vial with my diluent only ? And for *sample of known concentration*,...
27 July 2023 7,806 2 View
Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...
23 July 2023 974 6 View
If you can change your lifestyle/behaviour, how soon can these changes be detected in the epigenome? How long (estimate) would this change be detected in the majority of cells? Can this change...
22 July 2023 3,946 1 View
I want to screen for antimicrobial activities of spices extract against fungal isolates from food. what method /methods is best for it?
21 July 2023 162 1 View
How do bacteria decompose waste and why are decomposers necessary in the environment?
21 July 2023 6,651 5 View
Hello, I am bachelor student. I extract DNA from urine sample using two different DNA extraction kits and detect alpha thalassemia 1, SEA deletion type by real-time PCR. I use the same of pcr...
21 July 2023 9,337 4 View
What are the advantages of the method of counting bacteria with filter paper and in what cases is it mostly used?
20 July 2023 9,631 3 View
Dear colleagues, Do you know how moisture, colour and luminosity (e.g. CIELab) affect the shelf life of cosmetics products like soap, facial cream, shampoo, etc? Do you know any regulations...
18 July 2023 7,025 4 View
To generate the dispersion curve: 1. I use FR4_epoxy as substrate and Aluminium as dominos or corrugated surface. 2. Then I solve it with Eigen mode solver 3. I consider that the wave vector is...
17 July 2023 1,652 0 View
Especially related to nutritions.
15 July 2023 1,092 3 View
Can bacteria make their own energy through photosynthesis and bacteria used instead of water during photosynthesis?
14 July 2023 7,493 3 View
Is energy recycled while nutrients flow through an ecosystem and how do organisms contribute to nutrient cycling in the soil system?
14 July 2023 910 0 View
Why do microorganisms need nutrients to grow and how soil microorganisms facilitate the circulation of nutrients that are essential for plant and crop growth?
10 July 2023 7,188 0 View
Hi I need to ways that determine salinity and ph of microalgae dry powder To check the proper method of processing and washing microalgae and choosing the best method for drying microalgae that...
09 July 2023 5,365 0 View
If you know how to look the earth depth profile you will know how to look the earth lat-long profile
08 July 2023 8,551 2 View
Hello! We want to stain in our lab samples of Sempervivum infected by Endophyllum sempervivi. We would appreciate any suggestion of a cheap, quick and effective staining method to observe hyphae...
06 July 2023 5,994 5 View
Agriculture is the largest user of fresh water resources, consuming 70% followed by industry (20 %) and municipalities account for remaining 10%. Therefore, by reducing of agriculture water...
06 July 2023 7,056 4 View
in validating the total yeast and mold is it enough with 1 species representing molds and yeasts?
06 July 2023 8,063 5 View
Hi everyone, I am currently working with very low concentration of RNA and after Retrotranscription, I would like to concentrate cDNA by doing a PCR with the same random primers I have used for...
05 July 2023 6,323 1 View
How we can increase the conc. of solution after the desorption experiment. For example. doing the Adsorption process using activated carbon on a lab-prepared IBuprofen solution. Then after the...
03 July 2023 1,736 3 View