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Questions related to Extraction
Extraction of pesticides using modified QuEChERs method for fruits, food, vegetables and other oil samples the recoveries are not achieved at the spiked concentration levels ? Same issue in water...
24 February 2022 2,044 4 View
I have performed Tax4Fun analysis using the R program and got the output files with KO numbers and metabolic pathways. However, I could not find any tools for visualization and statistical...
23 February 2022 3,739 2 View
I need the appropriate temperature and duration for storing blood samples before DNA extraction
21 February 2022 5,374 1 View
Hello. My question is simple but it is indeed challenging practically. I have a water unstable compound (and acid unstable). It is an oxazolidine. Therefore, I cannot do simple extraction or even...
20 February 2022 2,164 4 View
A question about gas chromatography I'm using gas chromatography for my research. I am using gas chromatography in my research, and the peaks in the measurement results are rising in two steps. I...
18 February 2022 7,494 4 View
Hi everyone I am working on Stevia rebaudiana. I have gone through few research articles where water, methanol and ethanol have been used for the extraction procedure. Can anyone suggest that...
16 February 2022 6,559 5 View
Which lipid extraction method, is more suitable, from the given methods, for extraction of fats from Cheese sample. In which Cheese sample possess (~50% total moisture) Soxhlet Method Floch...
16 February 2022 244 4 View
Have you ever used a syringe/needle method for RNA extraction with Trizol? It would be in the homogenization stage. I read in an article that it would be better than homogenizing with beads....
14 February 2022 9,914 3 View
I am currently looking for a urease inhibitor that will not cross the lipid membrane of liposomes (DOPC. I suspect these molecules (picture attached) would cross the membrane but I can't find a...
14 February 2022 4,241 3 View
what would be the best method to extract the aloe vera gel using water, that would then be used in the antimicrobial activity experiment.
12 February 2022 1,164 4 View
Dear respected scientist, I need a alkaloids extraction method.
04 February 2022 7,326 5 View
Why is it important to have one area for RNA extraction and another for DNA extraction. is it because of the kind of reagents that are used for RNA purification that could be more volatile and...
03 February 2022 7,743 3 View
I want to transform in Chlamydomonas reinhardtii. Please guide me on which is the best strategy for transformation, Linear or circular vector. what is the difference between Linear and circular...
01 February 2022 7,491 0 View
Need for pathological study.
28 January 2022 8,496 1 View
When 100g dr weight was taken and using soxhlet method methanolic extract was prepared. The yield was 60g. I was quite amused by the values, is this high range value possible? Thank you
28 January 2022 9,237 4 View
Is it possible to do the aqueous extraction through supercritical fluid extraction?
27 January 2022 8,564 3 View
My goal is RNA extraction from yeast's ribosome; still now I made this with TRIsure but the result is terrible. Can someone help me?
25 January 2022 4,578 8 View
Good morning, does anyone know why buffered-saturated phenol is routinely used instead of Phenol:Chloroform:Isoamyl Alcohol in protocols for DNA extraction from low-melting point agarose? Thank...
20 January 2022 203 2 View
I'm using the vanillin-sulphuric acid method to cuantify saponins but the colour that I observe is yellow instead of red-purple. The method I follow from the literature is: - 1 ml of sample...
20 January 2022 2,885 3 View
As I'm a newcomer to metabolomic data processing from the very raw data, I was wondering how to combine pos and neg mode data together, especially in the condition of acquiring from separate...
19 January 2022 3,655 4 View
I wonder how the swab are processed in a very high throughput lab? is any automation system? What measures are for contamination prevention? Do need to cut the swab for DNA extraction?
18 January 2022 8,800 4 View
I am very new to the organic chemistry lab. Recently I have synthesized a product where I used Boc2O as one of the starting materials. In order to push my reaction forward, I had to add more Boc2O...
13 January 2022 670 6 View
Given the equation involving the W Lambert function. Eq.1) [1 + W(z0)]. {DW(z) in z0} = C/f(z0) with z0 a real number DW() is the first derivative of W or dW(z)/dz where DW(z) is Evaluated in...
11 January 2022 5,242 2 View
hi, Im doing DNA extraction from animal feces and two bands were obtained. upper band, and bottom band. but I can't explain upper band. (just below in gel groove) is it related with the...
06 January 2022 9,564 3 View