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Questions related to Extraction
Why for monolayer graphene I2D/IG value is 4 and for bilayer graphene it is 2? What are the physical significances behind this? All scientific answers and explanations related this are highly...
15 September 2024 5,985 2 View
Hi guys I'm going to extract RNA from cell culture and I have to put RNA-containing microtubes in the water bath for 10 minutes at 60°C. I want to ask if any Rnase contamination danger threaten...
14 September 2024 3,473 5 View
Modified hummer method or LPE in aqueous medium for graphene preparation need to be dried in Vacuum oven rather than binder ovens. Is there a significant reason for this vacuum drying?
03 September 2024 4,705 5 View
I fixed it with 4% paraformaldehyde and then stained it with DAPI.
29 August 2024 4,504 2 View
I am coupling TiO2 (which is full of OH groups) with graphene oxide (which also has a lot of OH groups). Therefore, by means of a condensation reaction, I am expecting that a bond of C-O-Ti...
18 August 2024 8,774 3 View
I recently fed mice with a type of stable isotope-labelled lipids and collect their tissues for lipidomics. The challenge is difficult to differentiate the effect of isotope on the identification...
15 August 2024 4,033 0 View
Hi, I culture HACAT mammalian immortalized cells. I am interested to check the protein changes by western blot and cell cycle analysis by flow cytometry. Can i store the harvested cell pellets in...
13 August 2024 9,034 3 View
5CB and hexane interact with each other in a very dynamic manner, but the interaction is complicated to understand besides Marngoni-driven and a few other basic mechanisms. For that, I need to...
13 August 2024 9,194 0 View
I want to know more about Uranium ore deposits in world.
13 August 2024 8,914 2 View
hi everyone I am having trouble in transfecting microglia N9 cell line. Now i decided to use Nepagene electroporator to transfect these cells. Can anyone send me a protocol for this as i am new to...
13 August 2024 6,088 1 View
I am using Materials Studio and the CASTEP software package. I would like to apply Hubbard U corrections to a model that includes transition metals. Should I directly adjust the Hubbard U values...
11 August 2024 6,165 2 View
Indiana University Bloomington (and all other IU campuses I believe) from is changing its email addresses from @indiana.edu to @iu.edu. Sometime this fall, all @indiana.edu emails will be sent...
29 July 2024 4,672 0 View
I am doing adsorption calculations on different surfaces of a metal using DFT, calculating work functions and adsorption energies. Is it Possible that a certain surface of a metal has higher work...
26 July 2024 2,845 1 View
The hidden wonders of Laos aren't just for tourists anymore. Research in this Southeast Asian nation is booming, and I'm itching to be a part of the next groundbreaking discovery. With my skills...
11 July 2024 2,652 0 View
Hello, I have a weird cloudy contamination that is appearing when I try to culture gamma delta Tcell in U bottom 96-well plate. The contamination always appears after a few days but does not...
26 June 2024 6,008 2 View
During the debridement process, larvae, such as those used in maggot therapy, secrete a variety of enzymes that primarily function to break down necrotic tissue and clean wounds. These enzymes...
20 June 2024 3,857 1 View
Hi, I am trying to make synthetic phage library. Right now , do people still use Kunkel method or other methods? Since it looks like Kunkel method is an old method. I am looking for more...
10 June 2024 6,922 1 View
I have a research paper on aircraft design but i wanted to publish it in a low impact factor scopus journal with low APC can anybody suggest me
10 June 2024 2,191 0 View
Where can i get the derivation of polymer screw extrusion process equation?
09 June 2024 1,718 3 View
I’m currently utilizing the ORCA software for my computations, applying the PBE0 functional combined with the def2-SVPD basis set and incorporating the RIJCOSX approximation. For the auxiliary...
08 June 2024 6,739 0 View
Hi y'all, does anyone know if animal cells increase in size as they age or do they simply multiply? I usually do a Percoll gradient centrifugation to isolate endothelial cells but with pups it...
08 June 2024 4,212 1 View
I have synthesized a gene specific primer for amplification of my target gene. However, I am getting amplicon of only 100 bp rather than 210 bp (target amplicon size). So far I have tested so many...
06 June 2024 1,730 7 View
Hi everyone, there is my issue: I try to synthetize nanoparticule of Chitosan according with this study doi: 10.1016/j.colsurfb.2011.09.042 I try it with HMW Chitosan (340-360kDa) but when I put...
30 May 2024 1,880 2 View
I want to find the values of h and s by manual calculating for X% each.
29 May 2024 6,998 2 View