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Questions related to Extraction
if a protein has low intracellular expression levels, it is likely that the amount of protein secreted into the extracellular space will also be low?
07 March 2023 6,712 6 View
Do you think that the medical profession has become inhuman and is commercial in some countries?
07 March 2023 5,927 3 View
How can we deposit a quality checked data sequence to NCBI?
05 March 2023 9,036 1 View
I have used the CMhyd for Bias correcting and downscaling of future rainfall and temperature projections with 6 different RCMs. I have an observed data period from 1985-2020 and I want to use it...
04 March 2023 2,402 0 View
I am working on DNA barcoding of herbal plants and dried herbs. I have tried several protocols like CTAB, SDS, and phenol chloroform extraction methods but the OD is always less then 1.5. i have...
03 March 2023 3,523 1 View
The effect of different growth stages stage 1, 2 3 and 4 and different concentrations of cadmium (0 ppm, 40 ppm, 80 ppm, 120 ppm, 160 ppm and 200 ppm) has to be checked on protein accumulation....
01 March 2023 1,304 4 View
I made an encapsulation of phycocyanin with whey protein isolate and i want to check the encapsulation efficiency. I tried to dissolve the capsule into distilled water to get the phycocyanin...
28 February 2023 8,473 0 View
We get a result from a company doing amplicon sequencing 16S rRNA for bacteria & ITS1 for fungi... the result report some yeast species by doing 16S rRNA.. is it possible.. or there any...
28 February 2023 2,444 3 View
I've seen recommendations to use it at 50°C for only a few minutes. Is that right?
27 February 2023 3,804 3 View
I reconstructed the phylogenetic tree using the Neighbor-Joining algorithm. The results showed that even though the species are the same (C.maxima), they are in different nodes or branches. What...
24 February 2023 7,351 4 View
I would like to extract bound phenolic in pigmented rice bran under alkaline and ultrasound condition. I followed the protocol using 2M NaOH for hydrolysis under ultrasound treatment and reducing...
21 February 2023 3,875 1 View
Need help with the DPPH antioxidant activity experiment In the first attempt, the blank was ethanol only. With an increase in the concentration of the sample, the absorption value was higher than...
20 February 2023 5,959 7 View
If extraction is part of the separation process, why are they called each by a different name?
20 February 2023 5,860 6 View
I did ethanolic extraction on my red seaweed using a soxhlet extractor. I'm planning to only reduce my extract from 250 mL to 60 mL. Then, I'm going to dilute it with 40 mL ethanol to achieve 60%...
16 February 2023 7,633 3 View
If we add soy-lecithin to liposome is it better for the post thawing motility?
16 February 2023 4,219 3 View
I am confused by the results of two-way ANOVA, and I'd greatly appreciate any help, please! For instance, I am collecting light intensity data for a 2-factor design: Season (summer, winter) and...
15 February 2023 6,635 5 View
I want some articles on the comparison of extraction and membrane-based separation process. As both are separation techniques can we do a techno-economic comparison? Need your views, Very...
07 February 2023 3,666 1 View
Hello, I am getting the error while converting a ligand sdf file to pdbqt format. How do I solve it? I got the error after I ran the following script mk_prepare_ligand.py -i L19.sdf -o...
31 January 2023 598 3 View
Hello, currently I am working on DNA isolation and during gel electrophoresis, DNA runs towards the negative electrode, Why?
28 January 2023 381 4 View
My research is on extraction of bacteria metabolites i need to use HPLC for identification of the metabolites
26 January 2023 7,321 2 View
I have received accession number data from NCBI, anyone can help how to create a Phylogenetic tree, I got all the data from the same organism and the same gene, I was thinking to make the tree...
19 January 2023 2,252 3 View
I have a 40-year time series with numerous dependent variables and just one explained variable to investigate the relationship between them. Which time series model can therefore be used in this...
19 January 2023 4,798 7 View
I am investigating the transport of gases. Would it be easier to extract a dissolved gas from another inert gas or from a liquid? What mechanisms could apply to practice the separation in the...
10 January 2023 4,163 0 View
Ultem material has the melting point is 426 degree Celsius. Is it possible to print Ultem material in FDM 3D printer. I am having Creality Ender 3Max type FDM printer. Is any other printers can...
10 January 2023 8,454 4 View