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The numerical statistical procedure of the statistical theory of Cairo techniques gives: Q=sqrt(T) Where Q is the quantum transition matrix of a quantum system. B is the transition matrix of a...
2 weeks ago 6,759 1 View
I am using Sorafenib on Huh7 and HepG2 cells. At 150 µM in 0.5% DMSO I observed something unusual in the drug-treated cells, not seen in the DMSO control. Could this be contamination or improper...
last month 4,607 2 View
In early experiment using anti-Mouse, my phage display worked well, but over time, when I tried to use the sample, I couldn't even get the phage precipitation and blue plaques when titered. So I...
last month 2,331 3 View
How do you select HEK293 cells after transfection without a microscope under the hood?
last month 1,025 3 View
What parallels can be drawn between Nokia’s late response to the smartphone revolution and the current risk faced by organizations or educators who resist integrating generative AI tools like...
03 July 2025 5,972 3 View
Hi every one, I am planning to do a research where I want to develop a protocol and later on pilot that, to develop, I will collect data through Nominal Group Technique. My queries are; 1. What...
04 June 2025 8,764 4 View
Therapists focus on issues in the wider relationship, which may make a woman more amenable to providing the intercourse a man wants. A woman may enjoy pleasuring her lover when she feels that she...
16 May 2025 9,187 2 View
The primers contain uracil. Primer Tms: 71°C (forward) and 63°C (reverse) I’ve already optimized annealing temperatures several times based on the lower Tm (63°C) but still get no visible bands....
29 April 2025 1,991 3 View
Need some good journals
28 April 2025 6,819 3 View
Please help me to generate ligand topology for the file attached here.
27 March 2025 5,770 4 View
Hi everyone, I am working on phage display and I have started panning process. I started with 10^15 phage titer for the first round and used 500ul of citrate buffer at pH 4 as a elution buffer and...
26 March 2025 8,340 4 View
I am encountering issues while preparing a PDB file of a substrate (UDP-MurNAc-L-Ala, with added charges and hydrogens) for molecular docking with AutoDock Vina. The conversion to the PDBQT format...
26 March 2025 7,983 3 View
There is some debate about whether RNA concentrations should be equalized before converting it into cDNA. Some scientists say this step improves the consistency of gene expression analysis using...
23 March 2025 4,938 3 View
need explanation about catalyst, time, solvent , workup procedure (acid or base workup),and purification methodology
18 March 2025 3,138 1 View
Hello, everyone. I isolated RNA from the cancer cell line utilising triazole and chloroform methods. I employed a Nanodrop machine to assess the purity of the extracted RNA and found the following...
17 March 2025 7,602 4 View
according to protocol that i am following in which i am using tris edta for washing, my dna samples are not transparent instead these are some creamy and also during running on gel electrophoresis...
11 March 2025 3,940 3 View
I am trying to view a .log file which terminated successfully using Gaussian 16 and get the following error: Unexpected EOF reading molecule specification I can view the .fchk files without any...
06 March 2025 9,828 2 View
Hi y'all I would like to know how to get a TFEA from a list of genes. I don't have sequences, but a list of DEGs from RNAseq analysis. I've only found one tool online TFCHEA3, but it's just for...
03 March 2025 1,129 0 View
In PyMOL, the RMSD calculation using the align command iteratively rejects atoms to improve structural alignment. The output reports multiple cycles of atom rejection (e.g., 44 atoms in cycle 1,...
02 March 2025 7,357 1 View
Dear Researchers, How can I calculate the DP for carrier relaxation time approximation (tau).
17 February 2025 1,114 3 View
Hi, I've been working with PC12 cells since 2022, and I never had a problem to attach them to a coated surface (I use PDL), but recently I started to use new vials, when I try to seed them in a 24...
13 February 2025 9,794 0 View
Has anybody ordered recently from ECGRC (The E. coli Genetic Resource Center)? Are they still active or does the site just exist as a place holder? It is formerly known as the Yale Strain...
12 February 2025 6,697 2 View
Research on interface and surface engineering to enhance the anisotropic properties of ternary magnetic materials has been a focus for years, particularly for applications in spintronic and memory...
10 February 2025 5,660 2 View
I'm trying to create compound-target-pathway (C-T-P) network in cytoscape. I started with two excel sheets (please see attached). First sheet has compounds and associated targets and the second...
10 February 2025 8,859 2 View