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Questions related to Extraction
Hello, Greetings. I would like to measure the recovery percentage of the organic pollutants by spiking the samples with certain working standard concentrations. My samples were air dried and...
14 August 2023 4,931 2 View
We have recently been finding that some of our LC-MS grade solvents have impurities (several brands) - notably acetonitrile and methanol. To avoid future problems, we would like to do an in-lab...
09 August 2023 834 4 View
For example, antibiotic X's MIC is reported as >32 µg/ml and MIC plant extraction is reported as >10 µg/ml. what value should we used to calculate the FIC according to the equation?...
08 August 2023 9,603 3 View
Hi! I need some help with the analysis of AAS results. We're analyzing the Ca and Mg content of soil and water samples. As means of determining the amount of metals in ppm (mg/L and mg/kg), we...
31 July 2023 5,819 3 View
Hi, my intern and I were working on imaging V. corymbosum buds in a confocal microscope (LSM900). Our plan was to have the same settings for all the images, but we changed (by mistake) the gain...
31 July 2023 8,987 2 View
I'm growing Pichia pastoris in BMGY medium currently and I'm trying to follow, how it grows, so I measure OD600. But as I expected the yeast to grow (and hence increase OD600), I diluted it more...
29 July 2023 9,294 3 View
Can a biofilm on a boat hull for example, corrode metal on one surface and be an attachment site for marine invertebrates on a different surface at the same time? Or does one event change the...
29 July 2023 3,097 5 View
I have tried multiple protocols with varying ratios of DCM and SDS but was not successful in preparing PLA emulsified Agar media. Problems faced , 1. Plates are too transparent to see any...
28 July 2023 804 2 View
Additionally, what factors should be considered when selecting an appropriate image processing algorithm for lineament extraction?
28 July 2023 4,906 3 View
For LOD and LOQ I need a *blank sample* and *sample of known concentration*. For the *blank sample*, should I just fill the vial with my diluent only ? And for *sample of known concentration*,...
27 July 2023 7,744 2 View
Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...
23 July 2023 909 6 View
I want to screen for antimicrobial activities of spices extract against fungal isolates from food. what method /methods is best for it?
21 July 2023 129 1 View
How do bacteria decompose waste and why are decomposers necessary in the environment?
21 July 2023 6,592 5 View
Hello, I am bachelor student. I extract DNA from urine sample using two different DNA extraction kits and detect alpha thalassemia 1, SEA deletion type by real-time PCR. I use the same of pcr...
21 July 2023 9,303 4 View
What are the advantages of the method of counting bacteria with filter paper and in what cases is it mostly used?
20 July 2023 9,563 3 View
Dear colleagues, Do you know how moisture, colour and luminosity (e.g. CIELab) affect the shelf life of cosmetics products like soap, facial cream, shampoo, etc? Do you know any regulations...
18 July 2023 6,980 4 View
Why do microorganisms need nutrients to grow and how soil microorganisms facilitate the circulation of nutrients that are essential for plant and crop growth?
10 July 2023 7,132 0 View
Hi I need to ways that determine salinity and ph of microalgae dry powder To check the proper method of processing and washing microalgae and choosing the best method for drying microalgae that...
09 July 2023 5,328 0 View
If you know how to look the earth depth profile you will know how to look the earth lat-long profile
08 July 2023 8,511 2 View
Hello! We want to stain in our lab samples of Sempervivum infected by Endophyllum sempervivi. We would appreciate any suggestion of a cheap, quick and effective staining method to observe hyphae...
06 July 2023 5,953 5 View
Hi everyone, I am currently working with very low concentration of RNA and after Retrotranscription, I would like to concentrate cDNA by doing a PCR with the same random primers I have used for...
05 July 2023 6,294 1 View
How we can increase the conc. of solution after the desorption experiment. For example. doing the Adsorption process using activated carbon on a lab-prepared IBuprofen solution. Then after the...
03 July 2023 1,684 3 View
Hi everyone, I'm currently doing DNA extraction for samples from the family Hesperiidae. I've done the extraction and my concentrations range between 1.5 to 4.98 ng/uL, which is quite low. I...
26 June 2023 2,356 0 View
I will like to know the best approach to this because of the biodegradative nature of these bioactive compounds.
24 June 2023 4,034 1 View