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Questions related to Extraction
I would like to simulate rectifier antenna using CST, is there any demo to do this?
06 January 2023 175 6 View
Since the TPC formula is cv/m, i want to know what sample volume v refers to. Is it the volume of the diluted sample or the sample we mixed when conducting the TPC assay?
05 January 2023 8,945 5 View
We have transfected HEK293 Cells with a viral gene. I have extracted genomic DNA from the mammalian cells. How can I determine the viral gene copy number using qPCR? Thanks in advance for the...
05 January 2023 9,298 4 View
Is there any good chemistry book about heavy metal precipitation/ extraction via chemical / chelate method?
04 January 2023 8,099 4 View
Hello, I actually would like an opinion, does onion epidermal cells is a good sample to do particle bombardment?
03 January 2023 6,504 1 View
The figure shows the between group comparison of the three group. This is plotted through SPSS on doing pairwise comparison in nonparametric analysis. Please help me in the description of this...
30 December 2022 5,356 3 View
Cytotoxicity of chitosan nanoparticles
28 December 2022 8,879 2 View
Recently, I used PVPP for the extraction of plant cells and some of them seemed to remain in the samples. When I tested these samples with LC-MS/MS, they were stuck in the column, leading to high...
26 December 2022 5,789 2 View
How do remove heavy metal ions from wastewater and what techniques would be best for separating a mixture of salt and sand?
25 December 2022 1,985 9 View
I have done the XPS analysis of ZIF-8 and ZIF-8-derived ZnO. In both, I got peaks at 281.5 eV and 282.4 eV respectively for ZIF-8 and ZIF-8-derived ZnO. What do these peaks infer? The binding...
21 December 2022 1,115 2 View
Is there any option (or procedure) to change the number of decimal places in the results and graphs in the SAS software?
21 December 2022 4,420 0 View
Hello RG friends, I have stained the pathogen infected plant leaves with DAB staining solution for confirming the H2O2 (hydrogen peroxide) accumulation. Successfully I got the expected result....
19 December 2022 4,051 0 View
I am isolating DNA from plasma samples of healthy donors but each time after measuring DNA on two different nanodrops, I get different DNA conc. One machine shows around 50ng/ul however other...
12 December 2022 1,349 3 View
It is known to all that the propagation of gravitational field is not instant. So for black holes, the gravitational field generated by the singularity of the black holes needs time to travel...
09 December 2022 3,217 34 View
Hello, everyone, In a study with 1 independent variable with 2 levels and 2 dependent variables, using MANOVA to analyze whether is right. If representing overall significant, post hoc comparison...
09 December 2022 5,044 3 View
Hello everyone, I would like to do UPLC. In the protocol there is 0.2% aqueous formic acid and 0.2% formic acid in methanol. what is aqueous means and how do we prepare them? Thank you for your help
09 December 2022 8,655 5 View
I have several specimens run to Aphis gossypii, but their longest hind femoral hairs are more than 0.9 × diameter of trochantro-femoral suture.
07 December 2022 6,668 0 View
I my research question there are two independent variable and one independent variable, first independent variable has two-levels (gender) second independent variable has three-levels and...
07 December 2022 2,888 4 View
I have a 5038 bp DNA fragment I am trying to extract from an agarose gel with little success. I am running a 50 cycle PCR with HF Q5 Polymerase and get a bright band of the expected size. I have...
06 December 2022 1,543 0 View
I have read literature but I am left with some doubts, like how much RNA is possible to obtain per cell? If it is possible to generate cDNA using the single cell without previous extraction...
06 December 2022 6,200 4 View
Dear Professors. I am very interested in sophisticate formulas involving the sum of several Li-functions in infinites terms, like C1*Li(1) + C2*Li(2)+... or others involving the polylogarithm...
01 December 2022 7,009 0 View
Currently doing basics of Molecular cell biology. And I have done a gel electrophoresis with DNA as part of RTPCR. Gel electrophoresis has mixes with DTT and one with Mg2+, which produced bands...
29 November 2022 7,638 5 View
Each of cell lines grows in a different medium. One in DMEM and the other in RPMI. Thanks
25 November 2022 4,610 3 View
Based on the Mediation model, I have 2 parallel mediators for which I would like to calculate the sample size based on a power of 0.80 and a medium effect size. Is what I had input correct?
20 November 2022 3,478 1 View