I searched the web for your query and found some relevant results. Here is a summary of what I learned:

• Amyloglucosidase (AMG) is an enzyme that can hydrolyze starch into glucose. It is produced by some fungi, such as Aspergillus niger, under certain conditions1.
• To extract and purify AMG from Aspergillus niger, you need to grow the fungus in a suitable medium, such as a modified Melin–Norkrans liquid medium2, and then separate the enzyme from the cell-free culture filtrate using various methods, such as ammonium sulphate fractionation, acetone precipitation, and chromatography3.
• To make a clarified extract, you need to remove any solid particles or impurities from the culture filtrate. This can be done by filtration, centrifugation, or using an aqueous two-phase system of polyethylene glycol and potassium phosphate1.
• A clarified extract can help to improve the yield and purity of AMG, as well as reduce the risk of contamination or degradation of the enzyme.
• Production of amyloglucosidase by Aspergillus niger using aqueous two-phase system. (2016). Retrieved from [ScienceDirect], a source of peer-reviewed scientific, technical and medical research.
• Abo-State, M. A., & El-Sayed, S. A. (2012). Production of amyloglucosidase by Aspergillus niger using some agricultural by-products. Retrieved from [African Journal of Biotechnology], an open access journal in the field of biotechnology.
• Kumar, A., & Singh, R. (2018). Extraction and purification of amyloglucosidase from Aspergillus niger. Retrieved from [International Journal of Current Microbiology and Applied Sciences], a multidisciplinary peer-reviewed journal.