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Questions related to Extraction
It has been reported there is more susceptibility of cardiac arrests and heart attacks early in the morning. What are the reasons for said higher risk.
18 November 2022 2,215 3 View
I have a problem when running CST . The errors are: Adaptive port meshing failed. Port mode processing failed. file locked (wb,16) I have increased the number of mesh cell to 20 and still having...
15 November 2022 9,032 0 View
I am planning to use youtube comments with my students to train them in thematic analysis. If our pilot study goes well, we might use it to present a poster at the university's conference. However...
12 November 2022 3,291 3 View
Hello, Can I by any means open .raw files that open by XCalibur by DataAnalysis Bruker Daltonics software ? Thank you
12 November 2022 6,508 1 View
I have DNA isolated from Leishmania parasites using a Qiagen kit. Please guide me how to lyophilize this DNA sample.
09 November 2022 290 2 View
I am trying to figure out how to make more TOPO vector. I'm using the PCR4Blunt-TOPO vector which is already linearized. In the kit there are only 25 ul of 10 ng/ul TOPO vector and I have do to...
09 November 2022 4,526 0 View
In sequencing using nanopores by MinIon, how many long reads per barcode and with what determined N50 are necessary for the assembly of a microorganism?"
07 November 2022 8,987 0 View
I have some flyash and want to leach out Al2O3 and SiO2 using NaOH. IS there any additive which can help me to improve rate of leaching or dissolution .
06 November 2022 4,941 2 View
Hello everyone, is there a generic rule in simulation setup of metamarials cells when it comes to what should be at the bottom of the substrate? Thanks for your responses.
04 November 2022 2,374 1 View
Disposable columns from NucleoSpin® Gel and PCR Clean-up can we able to reuse for 16s rRNA gene without affecting the quality of the product?
04 November 2022 660 2 View
Hi, Dear researchers, spike protein and nucleocapsid of SARS-CoV-2, which preferred to be targeted by drugs? Why?
31 October 2022 7,060 1 View
Between the shape of the well (flat bottom, bottom with minimal rounded edges, V-shaped bottom and U-shaped bottom), the material (polystyrene, cyclic olefin copolymer and Black microplates) and...
29 October 2022 9,291 2 View
Which stages in the QuEChERS extraction and clean-up technique are the most stable for storage over several days or weeks (e.g. when extracting polycyclic aromatic hydrocarbons (PAHs) from fish)?...
29 October 2022 2,369 0 View
Dear all, I really need your help, i am working with genomic DNA extracted from actinobacteria, i need fragments over 400-500pb and i have a sonicator to do that work, but i don´t know the best...
27 October 2022 178 2 View
Hi everyone, I am using a new LCMS with a c18 column. I am at the moment analyzing samples dissolved in PBS. To analyze them, I have to extract them with acetonitrile to solubilize my lipophilic...
19 October 2022 6,643 5 View
I've got a Roche LightCycler that I use to run my qPCRs. It used to take 1h 37 min to run a qPCR but now it take 1h 45 min using the same exact settings. Could the problem be that the machine is...
18 October 2022 9,057 3 View
I am going to do a research on post-treating wastewater by means of adsorbent pervious concrete. Because we have limitations on measuring heavy metals, it is possible to measure 2 or 3. I was...
17 October 2022 6,040 17 View
Hi everyone. I am working on making RNA probes for in situ hybridization, and am at the moment about to transform some competent cells. However, I just realized that the concentration of the...
11 October 2022 5,630 1 View
Can anyone explain a detail method to determine superoxidase dismutase (SOD), peroxidase and catalase activity from roots? Thanks!!
07 October 2022 7,146 3 View
Specifically, does anyone have experience of working with either or both Shimadzu Performance and Bruker Autoflex MALDI instruments and what is your opinion of instrument performance, servicing,...
04 October 2022 5,250 0 View
Hello RG Hivemind, For RNAseq of Aspergillus (or other filamentous fungi) with Oxford nanopore MinION, I am wondering: 1. What is the expected percent yield purified mRNA from total RNA? From a...
16 September 2022 9,003 1 View
My team and I are doing a simulation on Aspen for CBD oil, and we cannot literature on how to input CBD oils in Aspen or an actual compound in Aspen.
16 September 2022 854 0 View
I have stored my samples in 50 % glycerol for two months, I am now ready to do the DNA extraction using Bioline Isolate II genomic DNA kit. How does the 50 % glycerol affect the DNA purity or...
09 September 2022 4,365 0 View
Antioxidant Assay of medicinal plant with methanolic solvent.
09 September 2022 4,756 2 View