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Questions related to Extraction
The reducing sugars were estimated by DNS method.
01 November 2023 10,032 0 View
After trying different protocols I was able to extract RNA from old olive tree leaves with a phenol / chloroform : IAA protocol. Nanodrop measurments of the samples gave 260/280 rate ranging...
31 October 2023 7,950 4 View
Greetings. Im searching for protocols for utilization of solid state bioreactors, liquid state are the know standard so information for solid state is more scarce, anyone recomends articles or...
30 October 2023 4,797 0 View
can some one provide link to medical summary dataset (large) which is unstructured ?
30 October 2023 6,211 2 View
Microplastics
29 October 2023 3,826 3 View
My research requires answering this question.
29 October 2023 4,270 0 View
i am doing a research about optimization of assay probe-based (mutant labeled FAM and wild-type labeled HEX) and while doing the optimization of concentration of probes in duplex reaction for...
25 October 2023 5,846 0 View
Hi, I am currently trying to isolate immune cells from mouse colon tissue for my project. For this purpose, I am using a protocol which includes ; - Surgical removal of the colon tissue from...
23 October 2023 2,301 1 View
I have 11 samples done each in triplicate, and want to test if the correlation b/n different parameters are significant. So should I use mean values (n=11) or triplicate data (n=33) in Pearson...
22 October 2023 2,743 5 View
I have optimized the extraction of polyphenols using BBD via RSM. I carried out the extractions in triplicates and performed TPC assay on them. Hence, for each run I have 3 extraction with 3 TPC...
19 October 2023 1,353 0 View
Hi all, I am currently searching for articles about the structure of SRBSDV in tomo, but I have only found a few articles describing a part of the protein of this virus, and I have not found any...
17 October 2023 2,947 1 View
Pectin is freely soluble in water, but at the same time, Eudragit is insoluble in water. Hence need a common solvent for solubilizing both.
16 October 2023 8,196 3 View
Hi everyone I work on the structural analysis of a small DNA-protein complex (~150 KDa) using cryoEM. After reconstruction of this complex from purified proteins and DNA (42 bp), the complex...
16 October 2023 5,969 2 View
I need to know what the binding capacity of these microwells is to perform some ELISA experiments.
14 October 2023 9,313 2 View
Hello! I am recently got approval from my institution to carry out a quasi-experimental study to improve facility-based delivery in my state. I am looking for funding opportunities for this...
12 October 2023 5,765 0 View
In reference of high value of liquid extraction
12 October 2023 5,472 0 View
Greetings. Im working with PHB extraction and im interested in the use of ammonium laurate as described in this article, but the article itself is scarce on all details, a general protocol would...
12 October 2023 7,821 0 View
For RDE, 01. How to calculate the electron transfer number (n) from the Levich plot. 02. How to calculate the rate constant (k) for the kinetic current (jk) equation. 03. How to calculate the...
11 October 2023 10,087 1 View
Where is the list of UGC approved pharmacy journals available?
09 October 2023 8,195 3 View
Hi all, so i have data from the field collected at two different distance from the coast and for each station i have six size classes and respectively abundance. I wanna test the difference...
07 October 2023 9,174 4 View
I needed some guidelines for obtaining spray dried plant extract powder. I have using 50 % aqueous ethanol as my solvent and have performed liquid extraction from my plant using shaking incubator....
06 October 2023 5,625 0 View
RIPA is the best way to get the membrane bound proteins right? But has anyone tested Trizol isolated proteins to see if we get membrane proteins?
04 October 2023 2,417 3 View
Hi hope you are doing well it is to be stated that as a research engineer i am doing research on aluminum hydroxide and i did rection on lab scale from bauxite and caustic soda . I added milled...
04 October 2023 3,587 0 View
I have to conduct alpha amylase inhibitory activity but I m confused while taking readings from uv-vis spectrophotometer regarding blank and control. Do we have to zero the blank and then take...
02 October 2023 4,447 1 View