Extraction

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Questions related to Extraction

Wanna know the regulator mark point is medium or high for extraction of ethanol- toluene solution in soxhlet extraction of a hotplate?

Hi can anyone suggest the temperature range of a regulator for extraction of ethanol-toluene solution inside RBF Just using soxhlet extraction in hot plate? I mention medium or Hi in the regulator...

05 January 2022 9,804 3 View

Is anyone familiar with DNA extraction from Manure ?

when we use commercial kit for DNA extraction from soil we find nothing , we try to improve lysis step but we fail .anyone have a suggestions or advises. thanks in advance

31 December 2021 5,957 5 View

Can anyone give me the protocol of phenol-chloroform extraction of DNA from dried blood sample (Whatman filter paper)?

25 December 2021 8,714 10 View

Wax esters, phosphatidylcholine and sphingomyelin presents on a recombinant protein?

Hello to everyone, We are study a recombinant protein (Escherichia coli) which has a huge hydrofobic cabity in its structure. By Electron microscopy we saw a extra density in this cavity and we...

21 December 2021 7,695 2 View

Can any one help me in obtain bicine ( chemical substance) important for algal culture i need 0.5 gm with any price in egypt i can't wait import ?

Please help it's important for my work and i want 0.5 gm only with any price

10 December 2021 4,189 0 View

Optimize nematode extraction in forest soils?

Hello everyone, Is there any optimization procedure, in Whitehead 1965 tray method, to extract nematodes of hidrophobic soils such as forests? Best regards

06 December 2021 7,509 3 View

How to install softwares related to chemistry research on MacBook Air-M1 chip?

Hey! I've recently purchased MacBook Air with M1 chip.Can anyone help me out on installing free softwares such as mestrenova, Olex or Olex2 or wings, chemdraw, mercury, origin, ms office, mendeley...

03 December 2021 2,139 0 View

How to preserve the fresh tissure for nucleus and mitochondrion extraction?

Most commercial kits need fresh samples and the extraction must be done within a day. However, it costs 2-3 days for the samples to be delivered to the lab. Is there any methods that can store...

02 December 2021 8,650 8 View

DNA extraction following van der Beek protocol trouble?

I am doing a DNA extraction following the protocol of van der Beek et al. 1992. After the incubation at 65°C and posterior centrifugation, two phases are present plus the pellet; according to the...

24 November 2021 4,968 3 View

How to prevent ethyl acetate from degrading into acetic acid?

Dear researchers, I am currently using ethyl acetate as an extraction solvent during the workup of my lipase-catalyzed reaction. In this DSP: ethyl acetate is used first, for liquid-solid...

16 November 2021 3,060 4 View

Fatty acid extraction for gas chromatography but samples have glycerol in them?

I'm trying to run an assay on the breakdown on linoleic acid by certain enzymes. I ordered these enzymes from GenScript and they arrived in PBS buffer with glycerol as a cryoprotectant. From my...

16 November 2021 2,805 4 View

Hi, which one is better for metabolites extraction of seed, 70% Methanol or 100% Methanol then add 1 vol of water?

This is for the sample preparation technique for untargeted metabolite profiling by using LC-MS analysis. Thank you.

14 November 2021 5,148 4 View

Which methods are possibly used for the isolation of proteins from fish mucus?

Which procedures are mostly used for the extraction of bioactive protein.

11 November 2021 6,408 4 View

Is there anybody who know about the acidic extraction protocol for microbial metabolite?

I need the generally protocol🤷‍♀️

10 November 2021 1,323 3 View

Can digested tissues be maintained at -80°C before DNA extraction?

Dear all, can I store digested mice tails (which are digested with Proteinase K) at -80°C for a few days before DNA extraction? Will the DNA remain intact for a subsequent PCR? Thank you in advance!

09 November 2021 1,959 3 View

Before RNA extraction, how long can long non-coding RNAs stay stable in serum at -80 degrees Celsius??

Hello everyone, I am asking for the duration that I can store serum at-80 Celsius degrees before RNA extraction in order to detect their expression by RT-PCR without affecting RNA stability.

03 November 2021 5,158 3 View

How I can sperate DMAE from DMAE bitartrate salt?

Hello I want to assay DMAE. So I need sperate DMAE from its bitartrate salt. pleas help me to find best separation method. Thank you so much Razie #DMAE#deanol#bitartrate#DMAE bitartrate

03 November 2021 6,890 3 View

Hi there! Do anyone has experience in terms of efficiency of on column DNAse treatment vs post extraction DNAse treatment during RNA extraction?

I am doing RNA extraction from pea plant roots for sequencing purpose and require good quality RNA without contamination. I am using Purelink RNA Mini Kit for extraction. I found on column DNAse...

29 October 2021 2,342 0 View

What is main difference between geometrically modified groups and absorptivity profile groups in classification of heat sources? Which is the best HS?

Which is the best heat source among these for selective laser melting and why? Please give a detailed explanation. I referred to many papers but... what is the main difference between...

29 October 2021 3,797 0 View

How can I improve the posterior probability of my tree made in Beast 1.10?

I am doing some tests with covid data using genomes from the gisaid database, but frequently, after the analysis ends I get a tree with a lot of 0 values in the branches corresponding to the...

28 October 2021 9,502 2 View

Can you fully extract erucamide from polyolefin by using simple solvent extraction?

Me and my colleagues are looking for a quantative GC-FID/MS method for the determination of erucamide in polyolefin foil. Most of the articles suggest pressurized solvent extraction. We are not...

27 October 2021 4,833 1 View

What can be an effective protocol for extraction and transfer of Metagenome sample from soil to external sequencing facility?

I am performing a metagenomics based experiment, where I need to send the sample to an external facility for NGS. The transit is of approximately 7 days. What can be the extraction and transfer...

23 October 2021 660 2 View

Can I add more RIPA buffer to cell lysate if I didn't add enough during the initial extraction?

I tried running cell lysate from epidermal keratinocytes on a gel during sds-page but realized that it is severely contaminated with genomic DNA - I am fairly certain this is because I did not...

22 October 2021 9,122 1 View

Which is the best method for the extraction of alkaloids from Liquids?

Which is the best method for the extraction of alkaloids from Liquids such as soft drinks, juices, etc.

21 October 2021 9,871 6 View

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