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Questions related to Extraction
I want to quantify SCFA's present in mouse colon through GC-MS. Which form (neutral acid or anionic form) of SCFA's i should take as standard for GC. Also i will be performing derivatization of...
01 September 2022 5,522 0 View
Hi all, I am trying to purify ribosomal proteins. Does anyone happen to have a protocol? The first step is isolation of ribosomes, then extraction of rProteins. It looks like that the most...
20 August 2022 3,229 0 View
Many papers propose that sediment samples should be dried and sieved prior to sequential extraction, as suggested for soil techniques (e.g. Tessier or BCR). However, handling operations are a...
17 August 2022 3,301 0 View
Neglected tropical diseases in dermatology are scabies, madura foot, leprosy and others
01 August 2022 5,543 5 View
I'm having trouble getting males from the C. elegans strain hsp-4::GFP (SJ4005). I tried HS submitting L4 animals to different temperatures and times and also him-14 RNAi and neither approach...
29 July 2022 7,719 7 View
which process is better and easy
26 July 2022 2,795 2 View
I mean the unit of expresion
21 July 2022 740 2 View
What is the best statistical test to identify outcome difference between group1 and group 2; and each group has males and females. I want to test if there is any gender difference. I used Chi...
11 July 2022 1,866 5 View
I've got a protein that I need to reconstitute at 100ug/ml in PBS containing at least 0.1% of BSA, however in the CoA it says that it is supplemented with 50ug BSA per 1ug as a carrier. Do I need...
07 July 2022 9,380 1 View
hello I have a methanolic extract. Do I need to derivatize it prior or it's not necessary?
03 July 2022 8,228 6 View
I am preparing E. coli 70S ribosomes for cryo-EM and I would like to figure out the best buffer conditions. Looking through the literature, it looks like many groups use MgCl2 versus many others...
30 June 2022 1,702 0 View
I am interested in proteomic analysis of glioblastoma spheroids (diameter 200-300uM). How many spheroids should I grow to get atleast 3-5ug protein at the end of lysis? I will use M-PER...
29 June 2022 4,594 2 View
Thanks Alnajjar
27 June 2022 144 4 View
I have two groups - patients (n=60) and healthy controls (n=20), M:F appr 1:1 for now, I have half of the patients already. The distribution is such because the type of analysis (MLPA) and the...
20 June 2022 4,187 0 View
I am not getting the correct characteristic in reverse bias even after using impact ionization model. I am Attaching my code and result for reference. go atlas # MESHING SECTION mesh x.mesh loc=0...
15 June 2022 1,411 0 View
have UV-spectophometer absorbance reading through DNS method. I want to calculate sucrose and reducing sugars. I have also had UV-spectophometer absorbance reading of standard solution too. I need...
09 June 2022 1,996 0 View
I wish to make seaweed extracts and evaluate their antifungal effectiveness against anthracnose disease in bananas. Is it okay to use methanol and hexane for extraction because I plan to utilise...
24 May 2022 9,170 0 View
Can I use a whole blood sample maintain at a temperature between 4-8 degree Celsius for an enzyme assay after 24hrs?
06 April 2022 2,324 3 View
Is there a commercial ELISA kit for the measurement of saponin content? Thanks.
31 March 2022 7,511 5 View
I would like to concentrate fungal spores from a bacteria/spore liquid suspension, without having too many bacteria pelleting. Does anyone know which sucrose concentration would be the best? At...
22 March 2022 7,306 4 View
I am planing to make a comparison of forest cover between 1984 and 2021. I will use Landsat 5 and Landsat 8 images. Could some recommend any pre process corrections or calibrations for both...
19 March 2022 1,794 3 View
I am planning to do Soxhlet extraction of my plant material in Ethyl acetate and Methanol. Which type of chemical constituents are at the risk of decomposition while boiling in Ethyl acetate or...
06 March 2022 4,986 6 View
I gained results from extraction glycoprotein by a different solvent such as chloroform/ methanol (2:1), acetone, ethanol and ethyl acetate. I expected acetone or chloroform/ methanol to have the...
02 March 2022 2,074 0 View
I'm trying to remove DMF which is in my reaction mixture. I tried extraction with LiCl, EA, Brine solution already, Is there any better route ?
25 February 2022 611 3 View