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Questions related to Extraction
need explanation about catalyst, time, solvent , workup procedure (acid or base workup),and purification methodology
18 March 2025 3,192 1 View
Hello, everyone. I isolated RNA from the cancer cell line utilising triazole and chloroform methods. I employed a Nanodrop machine to assess the purity of the extracted RNA and found the following...
17 March 2025 7,668 4 View
according to protocol that i am following in which i am using tris edta for washing, my dna samples are not transparent instead these are some creamy and also during running on gel electrophoresis...
11 March 2025 4,012 3 View
Hi, Long story short, the HPLC didn't listen to the method batch and continued to run dry after pumping half a liter of water through it. It will have been sitting there for 5-6 days dry. Is...
08 March 2025 750 2 View
I am trying to view a .log file which terminated successfully using Gaussian 16 and get the following error: Unexpected EOF reading molecule specification I can view the .fchk files without any...
06 March 2025 9,893 2 View
Hi y'all I would like to know how to get a TFEA from a list of genes. I don't have sequences, but a list of DEGs from RNAseq analysis. I've only found one tool online TFCHEA3, but it's just for...
03 March 2025 1,204 0 View
What underlying physiological mechanisms contribute to the drought avoidance observed in early flowering genotypes?
03 March 2025 2,222 0 View
In PyMOL, the RMSD calculation using the align command iteratively rejects atoms to improve structural alignment. The output reports multiple cycles of atom rejection (e.g., 44 atoms in cycle 1,...
02 March 2025 7,417 1 View
Good morning, If any can explain how to use Multi-component density functional theory (MC_DFT) method for hydrogen nuclear quantum effects in Gaussian 16?
23 February 2025 7,170 0 View
Dear Researchers, How can I calculate the DP for carrier relaxation time approximation (tau).
17 February 2025 1,185 3 View
Hi, I've been working with PC12 cells since 2022, and I never had a problem to attach them to a coated surface (I use PDL), but recently I started to use new vials, when I try to seed them in a 24...
13 February 2025 9,860 0 View
Has anybody ordered recently from ECGRC (The E. coli Genetic Resource Center)? Are they still active or does the site just exist as a place holder? It is formerly known as the Yale Strain...
12 February 2025 6,752 2 View
Research on interface and surface engineering to enhance the anisotropic properties of ternary magnetic materials has been a focus for years, particularly for applications in spintronic and memory...
10 February 2025 5,737 2 View
I'm trying to create compound-target-pathway (C-T-P) network in cytoscape. I started with two excel sheets (please see attached). First sheet has compounds and associated targets and the second...
10 February 2025 8,926 2 View
I need a SCF.in file sample of full Heusler using GGA+U
09 February 2025 2,666 9 View
I want to find GDIS source code. Is there any way to compile it for windows??
09 February 2025 8,209 1 View
Dear researchers, I am new to Gwydion program and also new to magnetic domain size estimation from the MFM images. I would appreciate if you could teach a beginner like me using Gwydion to...
27 January 2025 3,472 0 View
Please help me find antibodies to detect N-terminal (NC1) and C-terminal (NC2) domain of the type VII collagen. Merck offers several antibodies clone LH7.2, giving contradictory information about...
23 January 2025 618 0 View
If we perform magnetic measurements of magnetic materials then what will be their applications in industry and in market?
18 January 2025 3,289 1 View
When conducting tests for gold extraction from ore, MS-test is used to show the dependence of gold recovery on its yield, maybe this test has another name but it is not GRG test
09 January 2025 9,159 0 View
"How can I ensure that my RTX 3090 GPU on a supercomputer is fully utilized for Abaqus 2023 simulations? Despite configuring the system, it seems the GPU is not under significant load during the...
06 January 2025 3,676 1 View
In the critical analysis of magnetic materials to find out the critical exponents, it has been seen in the papers that first construct plot a modified Arrot plots for different beta and gama...
03 January 2025 3,815 1 View
1. Is algae culture environment requirement different from typical PTC? 2. As per my reading of some of the papers, in case of freshwater algae, it is recommended to use glutaraldehyde, instead of...
29 December 2024 8,410 2 View
I want to add my publication. Is there any way you suggest my article? I remember you have system to show me the candidates.
10 December 2024 2,569 1 View