Extraction

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What's your idea and suggestion for my recombinant protein expression and purification?

Hello everyone, I'm currently trying to overexpress and purify a human hormone that is a member of the TGF beta superfamily (536 a.a without first 24 a.a signal peptide) with 7 cysteine amino...

22 June 2023 1,525 4 View

Can carbon based wastewater sludge be efficient as an adsorbent to remove contaminants from pharmaceutical wastewater?

preparation of wastewater sludge as a biochar to treat the above question.

19 June 2023 639 1 View

Can I get open research problems in STEM relative to computer vision?

What are some open research problems in the field of STEM with a possible CV solution?

18 June 2023 1,005 2 View

What is the difference between a covariate and a confounder?

what is the difference between a covariate and a confounder?

16 June 2023 6,934 3 View

Has anyone worked with aedes aegypti larval protein extraction? Which was the extraction protocol?

Use two types of buffer and the proteins are observed to be degraded. Am I doing something wrong?

14 June 2023 2,284 2 View

How to perform the metabolite annotation from AcquireX data?

Hi MS Enthusiasts, I am encoutering the issue regarding the analysis of AcquireX data for Metabolite annotation. As you know, AcquireX (from IDX orbitrap), provided by Thermoscientific, allow...

12 June 2023 7,204 0 View

Gut virome (Both DNA and RNA virus) extraction protocol?

Hello, I am currently in the process of designing a virome extraction protocol for fecal samples obtained from transgenic mice, with a specific focus on the gut virome. I would greatly appreciate...

12 June 2023 6,819 0 View

Evaporation of HCl after acid leaching of fly ash?

I did digestion of fly ash by HCl. Is it possible to evaporate HCl from filtrate? We don't have facility for evaporation of HCl by oven. Is there any chemical method?

12 June 2023 6,013 1 View

How to prepare a blank wash vial for HPLC?

I am new to HPLC and will need to prepare a blank vial to wash in between running samples. What is the typical protocol for how to prepare a blank? Thanks in advance!

10 June 2023 8,315 3 View

Immobilize biotinylated antibody on streptavidin magBeads?

Hi, I'm trying to stimulate human T cells with magnetic beads coated with antibodies. I have streptavidin-coated beads as well as biotinylated antibodies. According to the vendor, they usually...

09 June 2023 6,902 4 View

Amino acid analysis: Ninhidryn vs GC-MS ?

We extracted free amino acids (aa) from leaf tissue, using the 80-60-40% alcohol extraction method. Then quantified aa from the same samples by the Ninhidryn method and by GC-MS. The quantitation...

07 June 2023 1,336 1 View

How to rectify error in abaqus?

how to remove THE MATERIAL OPTIONS *CAP PLASTICITY, *CAST IRON PLASTICITY, *CLAY PLASTICITY, *CONCRETE, *CONCRETE DAMAGED PLASTICITY, *DRUCKER PRAGER, *FOAM, *CRUSHABLE FOAM, *MOHR COULOMB,...

07 June 2023 3,658 0 View

Ideal technique to separate microplastic (PVC) from culture broth ?

I have added PVC to my bacterial culture broth to see the degradation potential of bacteria toward the microplastics. The PVC, I have used is small powder granules. After proper incubation, I...

05 June 2023 6,412 2 View

"Nymphayol" belongs to which category of the Phytosterols ?

Dear Research Scholars, Could you kindly tell me that the "Nymphayol" belongs to which category of the Phytosterols ? (Stigmasterol or Beta-Sitosterol,..etc) Does anyone know about this? Thank...

30 May 2023 8,080 0 View

Molocular docking -results validation?

I did molocular docking of a small liberary of falvinoid ligands about 700 molocules , traget macromolocule Clacenurin A 4OR9. Using PyrX. Protein was prepared by Discovery studio . I get few...

26 May 2023 8,139 2 View

Kindly suggest extraction methods for bioavailable As in acid soils?

Kindly clarify whether the Soil As extraction method using 0.5M NaHCO3 at pH 8.5 is suitable for acidic pH?

20 May 2023 9,611 1 View

Is it correct to take the initial DPPH solution used as the A0 value in the DPPH radical scavenging test?

Is it correct to take the initial DPPH solution used as the A0 value in the DPPH radical scavenging test? For example; When 1 ml of sample solution of known concentration is added to 3 ml of DPPH...

18 May 2023 1,429 3 View

How to model and perform MD simulation of glycated protein?

I am interested in performing an atomistic molecular dynamic simulation of a protein in its native and glycated state. The glycating agent of my choice is methylglyoxal. However, I am unable to...

16 May 2023 9,054 0 View

Why the Navier-Stokes equations exhibit material-frame indifference for two-dimensional flow?

According to the book written by Pope, the vorticity equations exhibit material-frame indifference when the flow is two-dimensional and the rotations of the frame are steady. But how can we judge...

16 May 2023 4,757 3 View

How can I design the miRNA primers for RT PCR?

Hello everyone, I would like to ask a question to manage my experiment. To decrease the budget of my experiment, I have been searching for designing miRNA primers for RT PCR. I could find just a...

15 May 2023 9,551 4 View

How to calculate concentration from optical density for unknow pigment extracted from fungi?

how to calculate concentration from optical density for unknow pigment extracted from fungi?

11 May 2023 3,959 0 View

Extraction of polyphenols?

Hello, Many researchers reported that to extract polyohenols you need first to remove lipids. I don't know why? Knowing that removing lipids can leads to a loss of polyphenols even if the solvent...

10 May 2023 355 5 View

Which software can simulate solvent extraction process for separating cobalt from nickel?

I have effluent charged with cobalt and nickel and I need to simulate the liquid liquid extraction using software to know the effect of parameters such as O/A ratio, pH.... Many thanks

09 May 2023 6,787 3 View

Are there any studies that show the impact of the implementation of traditional medicine programs and policies on food and biodiversity?

The IPBES nexus is currently assessing the impact of indigenous health programs and policies on food and biodiversity. We are collecting articles, official documents, and grey literature...

08 May 2023 6,714 1 View

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