Analytical Chemistry

Analytical chemistry is the study of the separation, identification, and quantification of the chemical components of natural and artificial... | Contact experts in Analytical Chemistry to get answers

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Questions related to Analytical Chemistry

What is the best way to identify whether a certain substance is present in a mixture based on Raman spectra?

Dear Colleagues, our imaging facility is entering the field of Raman micro-spectroscopy and we've been approached by a group who want to detect the presence of certain compounds (about 10...

08 January 2021 912 5 View

How to identify possible metabolite from chromatogram?

We have done the analysis for a pre-clinical study and we have observed additional peaks in the chromatograms. We think that these peaks are metabolites. The concentration of the additional peaks...

02 December 2020 6,207 10 View

Alkaline hydrolysis and extraction of Vitamin D from food samples?

Hello everyone, may I ask about the purpose of adding ultrapure water after incubation for 30 minutes at 80 degree Celsius (under saponification process of vitamin D? Secondly, what is the...

02 December 2020 8,602 4 View

Acesulfame Potassium and Acesulfame and Conversion?

Dear to whom it may concern, Based on ChemAxon, I calculated pKa values of Acesulfame as follows: 1. pKa (Strongest Acidic): 3.02 2. pKa (Strongest Basic): -6 Because I would like to convert...

25 November 2020 4,673 3 View

Very small peptide detection in solution?

I am looking for an assay (rapid and convenient) where I can quantify/semiquantitative amount of very small peptides (dipeptides to tetrapeptides) in solution. I have tried different commercial...

09 November 2020 6,292 6 View

Bisphenol A in water - measuring bpa protocol using a uv/vis spectrophotometer?

Hi, I'm interested in a protocol for measuring concentration of BPA in distilled water (no interference) using a uv/vis spectrophotometer . The method found...

09 November 2020 7,129 3 View

Which parameter is the most important, removal % or adsorption capacity ?

I am testing a new material as adsorbent for the removal of methyl orange dye from aqueous solution, I got the below results, do I need to go further with higher concentrations even the removal...

05 November 2020 3,151 10 View

Difference between Porous Graphitic Carbon and Graphitized Carbon Black?

Dear to whom it may concern, May I ask you about the difference between porous graphitic carbon (PGC) and graphitized carbon black (GCB) in terms of surface properties, retention mechanisms, and...

10 October 2020 4,566 3 View

How to get rid of a constant peak at fix RT every time in HPLC ?

I am running catecholamine standards in HPLC using UV/FLD detector. I am getting a constant peak at fix RT (retention time @ 3.5 min, image enclosed) every time, even if I run another standard or...

29 September 2020 934 7 View

The quantifier for both analyte and internal standard have same product ion, will that have an impact on overall analysis?

Dear Chromatographers, This method was developed on LC MS/MS. The quantifier for both analyte and internal standard have same product ion, will that have an impact on overall analysis. Any...

31 August 2020 1,309 9 View

How to deal with negative values of autoradiography scans when plotting a calibration plot?

I am quantifying spots on an autoradiography scan, so subtracting the background pixels to get the intensity values but when nothing binds, it's the same as the background and since that's an...

18 August 2020 3,332 4 View

Why is ashing of certain organophosphorus compounds problematic?

When subjected to conventional ashing (i.e. residue on ignition through use of a furnace and noble metal crucible), compounds ArC(=O)P(=O)Ar2 [Ar = aryl] give ash values of a few percent %w/w...

12 August 2020 768 3 View

What is the best way for calibration curve for ternary system?

I'm doing experimental liquid liquied equlibrium of limonene +water+ ethanol, methanol and 1-propanol.. using UV in the analysis .. what is the best way to do calibration curve to estimate the...

20 July 2020 7,057 2 View

Does the concentration of a sample affect the absorbance measurement in the visible region?

The two plots in the attached figures illustrate spectra for copper-polyethylenimine (Cu-PEI) and copper-polyacrylic acid (Cu-PA2) complexes. In the spectrum for Cu+1.7 ppm PEI (first plot) there...

10 July 2020 8,401 3 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,754 2 View

How to quantitate endogenous metabolites in plasma?

Hi all, Our lab is trying to look at the differences in metabolic profiles of control and HD (Huntington's disease) patients. We have a few fundamental questions on how we should quantitate the...

31 March 2020 1,333 2 View

What could be the reason behind the poor linearity and high blank response from ICP-OES analysis?

I've been running an analysis for Pb with 5 standards, blank and a CRM; the results have had extremely poor linearity (i.e. standard 1 is a positive, standard 2 is a low negative number, and so...

31 March 2020 8,388 7 View

Possible reason to change the color of carbon dots from greenish into blue after immobilization process?

Dear experts, Liquid carbon dots (CDs ) were synthesized with greenish color under UV-light. However, after mixing with PVA polymer, the color turned to blue with enhanced quantum yield (QY). what...

16 March 2020 5,389 4 View

Chlorophyll-a in water samples by fluorescence. Quenching in environmental samples but not standards?

Hello, Our lab is currently adapting a previous working method (designed for the Turner TD-700) for the quantitative analysis of chlorophyll-a in water by fluorescence to be used with a new...

16 February 2020 8,008 4 View

Which method is best for extracting chlorophylls a and b into extract?

I requirement to know the most trusty extractant for chlorophylls a and b.

08 February 2020 2,395 9 View

Which method is best for extracting chlorophylls a and b into extract?

I requirement to know the most trusty extractant for chlorophylls a and b.

01 February 2020 6,069 8 View

Chlorophyll-a in water samples by fluorescence. Quenching in environmental samples but not standards?

Hello, Our lab is currently adapting a previous working method (designed for the Turner TD-700) for the quantitative analysis of chlorophyll-a in water by fluorescence to be used with a new...

01 February 2020 2,980 4 View

What are the possibility getting recovery 90% in all the levels like 50%, 100% & 150%?

Hi all, I am working on a molecule which is highly soluble in water. During the process of developing assay method (by HPLC) this molecule I facing a problem. Based on the solubility, I have...

01 February 2020 9,307 4 View

Can i use roomb for clean an analytical chemistry lab?

We are dealing with dirt issue in our lab, our lab is an analytical chemistry lab, can use roomba for cleaning it.

01 February 2020 6,468 2 View

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