Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
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Hello, I stimulate Cows PBMC with LPS and with an anti-inflammatory (6 hrs before the LPS) and another case (6 hrs after the LPS) Incubation time 24 hrs Results : RT Pcr decrease in the...
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Does water have a role in increasing the effectiveness of enzyme antioxidants?
22 August 2023 4,976 4 View
What are the drought stress tolerance mechanisms in plants and mechanisms involved in water movement in plants?
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It is a common practice that Researchers use both conceptual and theoretical frameworks in one study. The difficulty however is that sometimes one is able to develop the conceptual framework but...
22 August 2023 2,111 0 View
Green synthesis of nanoparticles
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Dear everyone, I would like to inquire if anyone could kindly recommend references that can be quickly studied for the purpose of learning Principle Component Analysis (PCA). Your assistance in...
18 August 2023 5,438 8 View
For two years, my colleagues and I were responsible for the water birds census in 32 marine sites in the south of Iran. We are now interested in publishing this data in the form of a scientific...
18 August 2023 6,278 6 View
I paired a homozygote knock-out female mice with hemizygote wildtype male. The gene of interest is on the sex chromosome. I was expecting all females to come out as heterozygotes however they...
16 August 2023 6,383 1 View
When lysing E. coli for recombinant protein expression, I utilized a lysis buffer composed of 1M Tris-HCl, Triton X-100, glycerol, lysozyme, MgCl2, and a protease inhibitor cocktail. After lysing...
15 August 2023 8,427 4 View
Are these files only used by Petrel? What are SGY, ZGY, and SGY.VAL files? What is the difference between file size, speed, and metadata of these files? What are the use cases of these files?
13 August 2023 4,171 1 View
Hello, I need to analyze the pharmacokinetic properties of my compound using the OSIRIS property explorer. The compound's SMILES ID is: C[N+](C...
13 August 2023 5,242 0 View
I want to know about the mechanism through which biological nitrogen fixation occurs in photosynthetic green microalgae apart from previously reported bacterial associations. Like in axenic lab...
12 August 2023 3,207 2 View
I am trying to study the interaction of anticancer compounds with calf thymus DNA.
11 August 2023 5,423 0 View
Hello, for a part of my thesis work I´m doing a DPPH assay on a food product extract, and I´m running into a situation where the absorbance rate of DPPH+sample during the first measurements ends...
09 August 2023 5,455 5 View
Preprocessing was carried out in the following way. 1. Sample 20ml 2. Place the sample on a hot plate and evaporate to 1ml 3. Dilute with 4ml 2% HNO3 in 1ml sample from hot plate 4. Analysis of...
09 August 2023 7,986 4 View
I tried with different concentration using 1) mobile phase A: 0.1% formic acid in aqueous solution, and mobile phase B: 0.1% formic acid in organic phase (acetonitrile) 2) mobile phase: ammonium...
09 August 2023 8,509 0 View
I have five FASTA sequences
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Hi. I am trying to create ligand topology and parameter files using SwissParam webserver. I followed all the steps to create the .mol2 file available in SwissParam page:...
03 August 2023 7,283 5 View
Hello everyone, I'm trying to quantify the concentration of mNeonGreen by measuring the absorbance and using Beer's law (A = εcl). On the following site,...
29 July 2023 9,968 0 View
Can someone give me an orientation? I want to calculate properties including boiling of chemical species, melting points, and packing efficiency. Can I do this using ORCA software? If the answer...
27 July 2023 796 3 View
Why is the pyramid of biomass always inverted in shape in a pond ecosystem and pyramid of energy in an ecosystem always?
26 July 2023 5,227 3 View
Respected Scientific Community, I am facing an issue with reading data in .chk file that I have just transferred (using win-SCP) from Gaussian installed on supercomputer. I have tried the...
25 July 2023 7,485 9 View
I'm trying to assay different lipases and their kinetic parameters using Ellman's reagent and DMPTB as a substrate. I've tried to measure the rate of reaction using a microwell plate in a...
23 July 2023 3,490 3 View