Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
I am developing a multiplex qPCR assay for GMO detection and am considering using an oligoset originally designed for the 23S rRNA gene to detect a synthetic DNA template as an internal control...
15 April 2025 7,276 2 View
Hi There, I do a reaction that my starting material has the salt of carboxcylic acid and the primary alcohal. I use the triphosgene in acetonitrile and SOCl2. After the reaction is done, I remove...
15 April 2025 9,081 0 View
Nil
14 April 2025 6,784 2 View
What Digital Skills are Necessary for Graduate Students in Light of Technological Development? ما هي المهارات الرقمية اللازمة لطالب الدراسات العليا في ظل التطور التتكنولوجي؟ If you have research...
14 April 2025 6,638 4 View
I have been having precipitation issues with all-trans retinoic acid (ATRA). I want to use it to differentiate my neuronal cell line. Here's what I've been doing: I made a 32 mM stock solution of...
13 April 2025 1,595 0 View
And if so, are there relevant articles I can refer to?
13 April 2025 6,926 3 View
I am currently using the Montreal Cognitive Assessment (MoCA) as a screening tool to evaluate neurocognitive alterations among individuals with crack cocaine use (locally known as "Bouffa") in...
13 April 2025 5,565 0 View
I found a HDAC in mitochondria of MCF-7 and was thinking of directing my research hypothesis towards insulin sensitivity and glucose uptake. Is it good model to study insulin sensitivity?
12 April 2025 5,678 0 View
Hello everyone, Could you please help clarify the differences and provide guidance on the following analysis methods commonly used in surface analysis of electrode studies? BET...
12 April 2025 7,887 4 View
innovative HR in SMEs
11 April 2025 279 6 View
How do women learn what an orgasm is? Your parents aren’t talking to you about it. Where would you learn? I don’t know. Maybe they’re reporting orgasms just when they’re having a pleasurable...
10 April 2025 2,636 4 View
Hello, I would like to analyze quantitative PCR (qPCR) data using R rather than proprietary softwares. I can see there are several packages out there, but mostly they perform relative...
10 April 2025 4,475 0 View
Please mention me the T cell specific ligands which are internalized by T cells. omiting antibodies will be good. pls provide me the references too
09 April 2025 1,613 0 View
We are looking forward to have collaborators who can help us understanding the mechanism of a formulation which effectively works in clinical cases of neurogenic bladder/ detrusar underactivity/...
09 April 2025 3,233 1 View
Hi all, I'm looking for a way to safely evaporate diethyl ether from graphene oxide. Time is not a constraint. I was thinking of reapplying the graphene oxide in a thin film and air drying in a...
09 April 2025 8,086 0 View
I've performed OE-PCR to amplify two amplicons (2.8 kb and 1 kb) as a single fragment. I first perform 10 cycles of PCR without the primers to allow fragments with overlap regions of about 30-40...
09 April 2025 7,311 0 View
TBSHS concentration in mobile phase is 50mM. As per above method, mutiple peaks are getting on Hplc chromatogram, which are interfering to analyte peaks, how to reduce it?? also how to control the...
09 April 2025 7,664 1 View
How many times and at what speed should centrifugation be performed to examine a-amylase, lactoferrin, total protein and Ig-a levels in saliva? In addition, is it possible to freeze the samples at...
09 April 2025 7,363 1 View
I am planning to purify a cytokine, and my plasmid contains a lac promoter and a periplasmic signal sequence. I am currently deciding between using BL21 (DE3) and BL21 Star™ (DE3) for protein...
08 April 2025 6,658 3 View
Dear colleagues, I attempted to establish a cardiac fibrosis model in SD rats using isoproterenol (freshly dissolved). I administered subcutaneous bolus injections at different concentrations...
06 April 2025 7,267 1 View
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06 April 2025 405 0 View
I have requested many times earlier to correct the name of the first author on the article which is my work (Shaista Jabeen, First Author) but it has still not been fixed. Here is the link with...
04 April 2025 427 2 View
I need to suppress the cytokine production by neutrophils in vitro.
02 April 2025 851 1 View
I am kindly looking for an incubation experiment protocol on measuring the sorption capacity of carbon dioxide by biochar when a known amount of carbon dioxide is inserted and gas samples...
02 April 2025 4,217 2 View