Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
How can I design and oligonucleotide probe to identify a clone containing a newly purified enzyme. Given a specific amino terminal sequence pf the enzyme MNDHYWIK? Indicate redundancy by putting...
20 July 2021 6,671 2 View
I have prepared an SDS lysis buffer containing the following ingredients: sucrose 30% SDS 2% EDTA 50mM Tris-HCl 0.1M Should I store it at room temperature or 4 C?
18 July 2021 3,615 5 View
Hey everyone, I'm currently looking into ordering some mannosidases and ended up at that website called creative-enzymes.com. I would like to order the mannan endo-1,6-α-mannosidase from that...
15 July 2021 8,164 1 View
Dear All, I have designed and synthesized inhibitors for the enzyme Prolyl Oligopeptidase (POP). To test the inhibitory potential of compounds, I have performed POP enzyme inhibition assay...
13 July 2021 6,665 13 View
searching for sources of enzymatic assays after obtaining them recombinantly
10 July 2021 8,900 3 View
Hi All, I’m preparing a structure for deposition on the PDB; everything is ready but I got a red alert in the Refinement statistic page. The error is relative to refinement of Shell 8 and it says...
09 July 2021 8,688 2 View
I am conducting experiments about enzyme kinetics. And I am confused about data normalization. For example, Uv absorbance of the enzymic reaction is usually used to monitor the process. I have...
08 July 2021 6,790 2 View
Hi, I am a novice in the field of biochemistry. Thus, need inputs on what current feasible biochemical assays are used to elucidate the role of an enzyme in a biological pathway. Any related...
07 July 2021 7,084 2 View
I am isolating mycoplasma phages by "picking" an isolated plaque and then culturing in Mycoplasma broth. After eluting the viral nucleic RNA/DNA and running the enzyme digest there are no...
07 July 2021 5,368 3 View
hello Are the following conditions true for simulating the molecular dynamics of an enzyme in the blood? *Aquatic environment *Ph = 7 *Body temperature thank you
05 July 2021 5,103 2 View
Say you have an enzyme with 3 ligands: a substrate, a cofactor, and an inhibitor. They all interact and effect one another. If one was to conduct a 3-dimensional titration of these ligands, are...
04 July 2021 1,122 2 View
Hi, I need to find the dimension and size of protein using PyMOL ,is there any one who could help me in this regard? That how to find the size and dimensions of protein and separately its chains?
30 June 2021 3,923 7 View
I've read the minimum requirement for the journal to get into an evaluation process. But, unlike PubMed or DOAJ that states the minimum number of articles published, there is no such information...
29 June 2021 6,605 0 View
Please guide me how to investigate interaction between miRNAs and mRNAs. Is there any online tool to do it? Tanx
25 June 2021 6,276 3 View
In several models of phage infection there is the term 'lysis rate' (η). What is the procedure for the experimental methods for measuring it? I could find nothing on PubMed. Also, is there a list...
24 June 2021 9,640 4 View
I'm studying digestive enzyme supplements and a large proportion of them contain a wide variety of proteases (Serratiopeptidase, DPPIV-peptidase, pepsin and others) as well as some other, mainly...
20 June 2021 2,119 4 View
After a successful over-expression of a key enzyme in a target pathway, Surprisingly, we have been noticed that another key Enzyme that leads our target pathway into a non-target-branch has been...
13 June 2021 448 12 View
I was wondering if the methods of enzyme immobilisation reduce enzymatic activity. I am aware that everything dependes on enzyme kinetics and the type of immobilisation, ¿but do the enzymes...
11 June 2021 198 3 View
I want to study the activity of a mannosidase against a substrate using a 96-well plate. Following the protocol, the reaction lasts for 1h and I will end up with a absorvance value at 450nm. How...
09 June 2021 8,658 9 View
Hey everyone! Do you know of any strategy/technique that allows me to identify whether a group of archaea that I have inoculated in a medium are alive or not? Thanks!
09 June 2021 540 3 View
In one of my studies, I found that repeated exposure to silver in bacteria at a sub-lethal concentration induces efflux and beta-lactamase activity. The induction of the efflux system is...
07 June 2021 8,584 3 View
I have 5microgram DNA . I want to digest it with Nco1 and BglII enzyme thermoscientific through double digestion. I have not succeeded so far. Can anyone suggest optimal double digestion protocol...
06 June 2021 3,630 4 View
Cell membranes have PC, PE, PI and PS as common components. What is the saturated to unsaturated ratio ? I mean such as DPPC: POPC?
06 June 2021 6,404 1 View
I have superimposed the structure of an enzyme after ligand (Inhibitor) binding with said enzyme and got RMSD value 0. If any change in the protein structure may occur then how to determine this...
29 May 2021 2,161 3 View