Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
how much lipase taking for enzymatic assay
24 September 2022 7,465 2 View
Also, can aliquots of microsomal solutions be stored at lower temperatures (-80 degrees) for further use without compromising their activity?
23 September 2022 5,533 2 View
Hello Everyone, I had been trying to express a codon optimised viral gene (1905 bp) cloned in pET-28a in various bacterial expression systems such as BL21-DE3, Rosetta, C43, and BL21-pLYSs for...
17 September 2022 3,658 8 View
formula or methods of determination
16 September 2022 1,100 1 View
Medicarpin estimation requires a long protocol and HPLC. Is there any other way Medicarpin can be estimated? Your help will mean a lot. Thank you.
07 September 2022 579 0 View
I stored hydrogel open air for several days and it lost more than 50% of its weight. It is true that the main reason of hydrogel weight reduction is loose of its water content as hydrogel is...
05 September 2022 6,522 4 View
I want to conjugate a hapten via its primary amine to carboxyl groups on a carrier protein using EDC/NHS. I understand this is normally done by first activating the carboxyl-containing molecule...
05 September 2022 1,942 6 View
I am working on the effects of inhibitors, particularly of AlO and FeO on soil phosphatases. Currently, we want to test the effects of AlO and FeO on the activity of phosphatases. We only have one...
01 September 2022 4,896 2 View
Hello fellow researchers: This is generated using BIOVIA Discovery Studio. My question is: How could I move the numbers of distance to the position of my choice? As you could see in this picture,...
24 August 2022 1,569 1 View
I am culturing immortalised mouse embryonic fibroblasts; currently at passage 10. Today I noticed these long thread-like structures floating above the cells (see picture, 40x magnification). The...
21 August 2022 984 0 View
Circular Dichroism (CD) Spectroscopy for determine secondary structure of enzyme.
20 August 2022 7,068 4 View
Hello everyone, I have a question about flow cytometry and its procedure in order to analyze cell-surface markers. What would be the best blocker while the cell line and secondary antibody are...
18 August 2022 2,485 0 View
I have a protein molecule which is literally an enzyme and a small protein molecule which shows probable inhibition to the enzyme. Now the matter is that the mechanism is not clear that how the...
18 August 2022 2,010 0 View
Coenzyme q10 is available in Reduced and Oxidized form
18 August 2022 6,259 2 View
Plz helpe to get books or papers about Advantages and disadvantage of silver nanoparticles and zinc oxide compared with other metal nanoparticles
17 August 2022 1,591 3 View
I want to separate dead cells from a bacterial population after treatment of any drug or antimicrobial agent. Which technique will be more efficient FACS or MACS if I use PI and SYTO9 dye ? Is...
16 August 2022 3,996 3 View
How to study and determine the heavy metals attached to the organic part of the soil? can you tell me about any equipment or method?
15 August 2022 3,898 6 View
This is because I changed my mine and decided to extract RNA from half of the pellet and also I was running out of PBS so I tried water instead
13 August 2022 8,282 0 View
I am measuring the gene expression of my target gene DDX43. I took 2.5 ul of cDNA for Q-PCR. I am using SYBR GREEN for my q-PCR. The concentrations of RNA for most samples were 20-60 ng/ul. The...
11 August 2022 6,376 5 View
For example Times 0.5, 1, 2, 4, 6 hours and concentrations
09 August 2022 1,431 1 View
How can the researcher determine the stability of a site for collecting data?
04 August 2022 9,137 6 View
please help me with the question asked above .
28 July 2022 7,101 0 View
Is it accurate or false, and why?
28 July 2022 9,197 5 View
For my experiment I have immobilised a his tagged protein on Dynabeads his-tag isolation and pulldown magnetic beads that will be used to pull out specific cells from PBMCs. I need to...
25 July 2022 3,281 5 View