Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
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Questions related to Enzymes
By a 'higher differentiation capacity' I mean that it would be easier to differentiate them– especially without adding a pool of chemical factors. I think cell lines might be tricky as they are...
21 January 2022 5,332 5 View
Does anyone know what drug or method is suitable to remove the sulfate group from an intramembrane enzyme? Note that my goal is to work in vivo and I do not want cytotoxicity.
18 January 2022 3,746 4 View
Hello, There is an article titled "Anti stress activity of olea europaea". I have attached it to this question. Could someone please explain to me what is meant in this paragraph: "As a result of...
17 January 2022 1,197 0 View
I want to know how I would prep 500ml of 25% weight/volume potassium hydroxide. From this stock, how would I perpare a 0.1M working solution? If you could give as much detail on working out too...
15 January 2022 702 2 View
The significance of logfc is less than 1, and the p value is less than 0.05. is it statistically significant?
14 January 2022 8,683 5 View
The value of Ki calculated for competative and non competitive enzyme inhibitors. Normally I have got maximum activity for 'a' compound, means it has got least IC50 value. It followed non-...
12 January 2022 6,854 3 View
Hi my scientific family: Hopefully you are fine and well. I have inserted the pictures of SWISS Model for enzyme and used discovery studio to get molecular structure of swiss 3d diagram. I used...
10 January 2022 606 0 View
I am looking for a proper protocol for the Nitric oxide scavenging activity and according to the principle, the absorbance should decrease with increasing concentration, but I am getting increased...
05 January 2022 5,895 5 View
Several of my enzyme kinetics experiments have resulted in vmax increasing with an increasing amount of competitive inhibitor which is contrary to my expectations. What could have caused this? All...
27 December 2021 5,771 4 View
Can anybody brief me on the zeta potential of mRNA?
27 December 2021 8,826 4 View
I have estimated the amount of ammonia liberated using the Nessler method, but now how to calculate L-GLutaminase enzyme activity? Is there any formula?
26 December 2021 2,868 10 View
What is the percentage of purity of ethanol needed to be used in the purification process without any interaction taking place with the nanoparticles when separated? Ethanol diluted or...
26 December 2021 7,054 5 View
Hello, Several studies show honey as being anti-inflammatory, so I was surprised to see in this article: https://pubmed.ncbi.nlm.nih.gov/12824009/, that honey stimulated release of inflammatory...
25 December 2021 6,876 0 View
Looking to buy a data logger for microbial fuel cells. Anyone can suggest the best and economic data logger with multiple channels. Preferably Indian make or Indian supplier!
21 December 2021 1,546 1 View
I am planning to purchase treated bamboo fibers for my graduate studies.
15 December 2021 7,053 0 View
If the goal is to use the enzyme in detergents powder, how much purity is usually needed? Is it possible to use spray dryer just after one step precipitation of fermentation broth, to produce the...
13 December 2021 1,949 0 View
Hi, I have natural polysaccharides and their modified derivatives. I want to test them for antibacterial activity but some of samples are too viscous at 10mg/ml that they don't get into the tip of...
11 December 2021 7,692 7 View
I have osteoinduced my WJMSCs cells for 2 weeks. Now I would like to measure the level of ALP in the osteoinduced cells. Im not sure should I use the cell lysate or the culture medium to measure...
08 December 2021 5,632 0 View
Relation of CSF flow in relation to sleep and dreams is now will established. As well is the daytime flow of thoughts. By raising this question I am trying to open a discussion about another...
04 December 2021 6,335 11 View
I am trying to make fixative for transmission electron microscopy of our fibroblast, and for some reason my solution goes cloudy when it should be clear. I was given a protocol to work off of by...
04 December 2021 1,544 0 View
In my research, I have to determine the protease activity. so How can I convert tyrosine liberation in microgram/ml to micromole/ml and vice versa.?
03 December 2021 3,453 3 View
Dear all, I am currently searching for software that can date my RAxML tree data set (100 trees, ~3,000 taxa) . I already tried using treePL but encounter an error that I was not able to solve...
03 December 2021 1,294 2 View
I would like to validate the results of RNAseq but I am not familiar with this. I currently have a table with Gene IDs (Ciclev10004574m.g for example) and their Arabidopsis homologs (AT5G52300.1...
30 November 2021 8,490 2 View
Hi, I want to attach biotin-lysine at the C terminal of peptide sequence [DPVLWRHFILSGPGYK(Biotin)CONH2]. I read that 20% piperidine can be used for deprotection (Solid-phase peptide synthesizer)...
24 November 2021 2,336 5 View