Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
1,464 views 4,059 posts
Questions related to Enzymes
Hello researchers, I would like to ask whether it is necessary to include definitions in a survey questionnaire for a research topic where the target survey population may not be familiar with the...
08 March 2024 2,877 2 View
I am developing a protocol for the generation of antibodies using the Phage display method. Could anyone share a protocol for bio panning of biotinylated antigens?
06 March 2024 9,529 3 View
I need to know that after codon optimization what should be the GC content?
05 March 2024 7,041 1 View
Hi everyone, I need to know what solvents are commonly used, the type of solvents, their purpose, and their recommended concentrations. Any advice is welcome. Thank you.
04 March 2024 9,933 4 View
If I isolate a motif in a domain, is it still functional? Will the amino acid sequence function if I synthesize only the binding site region of the domain?
04 March 2024 918 4 View
I need to know how to store Arabidopsis roots for further analysis in a way that hair roots are preserved in their integrity, without degrading or collapsing, suffering from dehydration, etc. I...
01 March 2024 8,078 6 View
I'm doing RNA dot blot with NC membrane. But its seems like something wrong with crosslinking. I'm wondering 1. If I need to use SSC buffer to treat with membrane before dotting? and what X of...
29 February 2024 4,679 1 View
I want to perform MD simulation of the OprF porin along with the outer membrane of bacterial species Pseudomonas Aeruginosa in CHARMM-GUI web server. Now how do I have to select the composition of...
28 February 2024 810 0 View
Any HPLC assay method for thiostrepton
28 February 2024 3,770 1 View
I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.
28 February 2024 6,911 1 View
I'm currently working in the Trudeau laboratory at the Université de Montréal in Canada, renowned for its cyclic voltammetry for dopamine detection. After learning about this technique and...
27 February 2024 3,783 0 View
Greetings esteemed colleagues, I am currently working on my master's thesis, focusing on a protein product that requires the use of calcium chloride treatment in one of its stages to form calcium...
25 February 2024 1,776 2 View
I am trying to do hydrolysis of DCFH-DA with 0.01 M NaOH. Stock is dissolved in anhydrous DMSO. Both the solvent and base is degassed. The problem is dye seems to get hydrolyzed and oxidized at...
22 February 2024 5,988 0 View
I want to test out some new primers, but I know from past PCRs that at least one of my reagents is contaminated. I'm on a time crunch to finish my project and am not sure if I can get new reagents...
22 February 2024 4,763 0 View
I want to detect sodium lactate using HPLC. I used a C18 column and a mobile phase of 0.001M sulfuric acid solution. I set the stop time to about 8 minutes, but I'm not seeing any peaks. What...
21 February 2024 2,313 2 View
Can a newly developed questionnaire tool that is validated and checked for reliability be communicated to an Open Access journal?
20 February 2024 6,805 0 View
In case microbial samples, e.g. environmental water samples need to be stored or transported for flow cytometry analysis, what would be a good method for preserving integrity of cell membranes?...
15 February 2024 2,248 3 View
Optical coherence tomography (OCT) has become a highly valuable noninvasive imaging technique for studying the retina in vivo. However, the equipment required for OCT imaging is costly, and in...
14 February 2024 4,748 1 View
Although industrial stakeholders are one of the large producers of PFAS, it is important to note that they are not the only source of PFAS in the influent to a wastewater treatment plant. Consumer...
13 February 2024 5,141 0 View
Dear Madam/Sir I need a new complete reference about the effect of air pollution on plants/agricultural plants. I would be very appreciate if you share the reference with me. Thank you for your...
10 February 2024 6,330 2 View
How to perform seismic inversion using Petrel software?
10 February 2024 7,750 0 View
Jiangxi Province ChengZhu Environmental Engineering Company Ltd was incorporated in April 2015 in Ganzhou China, with Registration NO.91360700343188092B. The currently registered business scope in...
10 February 2024 4,084 1 View
What are some methods to measure computational thinking? What are the assessment tools for computational thinking?
09 February 2024 3,995 2 View
good day, I am conducting research on media images of (public) social welfare and media reporting of social workers. Can I ask for links to articles and studies what the media image of social...
08 February 2024 8,225 1 View