Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however... | Contact experts in Enzymes to get answers
2,314 views 5,360 posts
Questions related to Enzymes
Hello everyone. Maybe you are experiencing the same problem. For some time now (before everything was going well!) when I put new versions of my preprints online, the DOI is not updated (a new...
25 June 2025 8,554 0 View
In my lab, i am doing the in vitro transcription experiments to get our tRNA, i do the PCI-CI step, then refolding, but after refolding, i add the MgCl2 it becomes so cloudy. It's unlikely because...
24 June 2025 8,056 2 View
There are no papers at all on the synthesis of XM-19 SS, using HIP. if someone can share his experience or suggest some articles regarding the Temp, and pressur, would be quite helpful. Thank you
24 June 2025 4,415 2 View
Hello, for years it turns out my article was not searched on google scholar because the names of two different authors are fused to make name of one author on research gate. For example, instead...
23 June 2025 7,925 0 View
Have you ever read this article? Muñoz, Lucio, 2022. Sustainability thought 165: How can we show that the overpopulation framework a la ecological overshoot is a subset of the most distorted...
23 June 2025 4,538 0 View
My DNA stock concentration eventually decrease. I suspect my EB not containg EDTA and I want to make TE buffer. But, i just have Na-EDTA.
20 June 2025 387 1 View
My lab uses the primers from IDT but since I arrived here I noticed that when the primers arrived they work perfectly but then I started to notice that the bands in the gel become faint and after...
20 June 2025 395 0 View
How to practice religion without a personal relationship with a deity?
19 June 2025 7,732 4 View
Umbilical cord-derived mesenchymal stem cells (UC-MSCs) were seeded at a density of 3 × 10⁵ cells per well in a commercially available fibronectin-coated 6-well plate. Before differentiation was...
17 June 2025 9,709 2 View
Dear all, I’m conducting a meta-analysis on an intervention’s impact on learners’ emotions. My dataset consists of correlation coefficients for both positive emotions (e.g., enjoyment) and...
17 June 2025 7,440 3 View
Mijn artikel: Effects of burning and grazing on root biomass in the páramo ecosystem / A.I.G.A. Rossenaar and R.G.M. Hofstede. In Balslev, H. and J.L. Luteyn. Paramo. heeft op googelscholar 24...
16 June 2025 8,587 0 View
The 11th World Congress on Infectious Diseases (WCID-2026) April 15–17, 2026 Athens, Greece Dear Dr. Corrado Mollica, Greetings from the Organizing Committee of WCID-2026. I am Jodie Jiang, and...
14 June 2025 365 11 View
To study the effect of mesenchymal stem cells on rat model myocardial infraction condition, for which MSC can test Rat MSC or Human MSC?
13 June 2025 6,890 0 View
I have synthesized copper oxide nanoparticles using published methods for several times, but could not get the same size as reported in the paper using zetasizer. Metal precursor used was copper...
12 June 2025 342 3 View
Hello everyone, Does anyone know whether collagen-cell binding can somehow alter the fluorescence signal from collagen-FITC conjugate and why? I am working with plastic petri dishes coated with...
11 June 2025 4,633 3 View
Water beetle found in streams in the Tunisian Oasis. Plz send a message to Pr. Samir Touaylia
10 June 2025 5,376 4 View
I'm trying to transfect adherent human rhabdomyosarcoma (RD) cells with PX458 vectors using Lipofectamine 3000, following the manufacturer's protocol. Here's a brief overview of my procedure: I...
08 June 2025 9,961 2 View
Question: Dear researchers, I am currently working on Fe, Mn, Ni, and Fe/Mn co-doping in Co₃O₄ for the investigation of magnetic and optical properties using Materials Studio (CASTEP DFT...
07 June 2025 8,521 5 View
Hi. I'm currently culturing THP-1 (ATCC TIB-202) cells for my experiment and have been experiencing persistent clumping issues. I would greatly appreciate any advice or recommendations you could...
03 June 2025 5,176 2 View
I am using MboI enzyme to digest cross-linked 100k single cells (in situ Hi-C), but I have noticed that there are always large fragments present after the digestion (similar in size to the...
03 June 2025 1,835 1 View
The title is The relationship between sports activity and emotions in the formation of cognitive processes. It's from the journal Journal of Physical Education & Sport. I need to give a report...
02 June 2025 595 1 View
In our recent MTT assay using the MCF-7 cell line treated with Tamoxifen, we observed a surprising increase in cell proliferation at low concentrations (below 1 μM). Interestingly, the same...
01 June 2025 3,259 4 View
In the context of rapid digitalization, especially with the rise of social media, virtual workspaces, and AI-generated content, traditional notions of identity, belonging, and community seem to be...
31 May 2025 1,833 6 View
Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...
31 May 2025 6,634 2 View